Evidence map›Paper›PMID 31749347›Full record

ArticleJournal of Zhejiang University. Science. B

A novel oncolytic adenovirus inhibits hepatocellular carcinoma growth.

Yu-Huan Bai, Xiao-Jing Yun, Yan Xue, Ting Zhou, Xin Sun, Yan-Jing Gao

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of Zhejiang University. Science. B. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.3field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
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  4. Article
  5. Review
  6. Recent progress in treatment of hepatocellular carcinoma.American journal of cancer research · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Yu-Huan BaiDepartment of Gastroenterology, Qilu Hospital of Shandong University, Jinan 250012, China.
Xiao-Jing YunDepartment of Gastroenterology, the Second People's Hospital of Liaocheng, Linqing 252600, China.
Yan XueDepartment of Gastroenterology, the Second People's Hospital of Liaocheng, Linqing 252600, China.
Ting ZhouDepartment of Gastroenterology, Qilu Hospital of Shandong University, Jinan 250012, China.
Xin SunDepartment of Gastroenterology, Qilu Hospital of Shandong University, Jinan 250012, China.
Yan-Jing GaoDepartment of Gastroenterology, Qilu Hospital of Shandong University, Jinan 250012, China.
People’s Hospital of Linqing · CNQilu Hospital of Shandong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo evaluate the inhibitory role of a novel oncolytic adenovirus (OA), GP73-SphK1sR-Ad5, on the growth of hepatocellular carcinoma (HCC).

methodsGP73-SphK1sR-Ad5 was constructed by integrating Golgi protein 73 (GP73) promoter and sphingosine kinase 1 (SphK1)-short hairpin RNA (shRNA) into adenovirus serotype 5 (Ad5), and transfecting into HCC Huh7 cells and normal human liver HL-7702 cells. The expression of SphK1 and adenovirus early region 1 (E1A) was detected by quantitative real-time PCR (qRT-PCR) and western blot, respectively. Cell viability was detected by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay, and apoptotic rate was determined by flow cytometry. An Huh7 xenograft model was established in mice injected intratumorally with GP73-SphK1sR-Ad5. Twenty days after injection, the tumor volume and weight, and the survival time of the mice were recorded. The histopathological changes in tumor tissues were observed by hematoxylin-eosin (HE) staining and transmission electron microscopy (TEM).

resultsTransfection of GP73-SphK1sR-Ad5 significantly upregulated E1A and downregulated SphK1 in Huh7 cells, but not in HL7702 cells. GP73-SphK1sR-Ad5 transfection significantly decreased the viability and increased the apoptotic rate of Huh7 cells, but had no effect on HL7702 cells. Intratumoral injection of GP73-SphK1sR-Ad5 into the Huh7 xenograft mouse model significantly decreased tumor volume and weight, and prolonged survival time. It also significantly decreased the tumor infiltration area and blood vessel density, and increased the percentages of cells with nucleus deformation and cells with condensed chromatin in tumor tissues.

conclusionsGP73-SphK1sR-Ad5 serves as a novel OA and can inhibit HCC progression with high specificity and efficacy.

Indexed as

AdenoviridaeAnimalsApoptosisCarcinoma, HepatocellularCell Line, TumorFemaleLiver NeoplasmsMembrane ProteinsMiceMice, Inbred BALB COncolytic VirotherapyPhosphotransferases (Alcohol Group Acceptor)Promoter Regions, GeneticSphingosine KinaseGOLM1 protein, humanMembrane ProteinsPhosphotransferases (Alcohol Group Acceptor)Sphingosine KinaseHepatocellular carcinoma; Oncolytic adenovirus; Golgi protein 73; Sphingosine kinase 1

Identifiers

PMID31749347
PMCPMC6885404
OpenAlexW2991899139

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.