Evidence map›Paper›PMID 31749084›Full record

ArticleMolecular biotechnology2020

pGP-B2E, a Recombinant Compatible TA/TB-Ligation Vector for Rapid and Inexpensive Gene Cloning.

Dongyue Li, Chao Zheng, Jie Zhou, Bin Chen, Rumeng Xu, Wenxia Yuan, Ersong Zheng, Weifang Liang, Yong Yang, Lijuan He and 4 more

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Dongyue LiCollege of Plant Protection, Yunnan Agricultural University, Kunming, 650201, People's Republic of China.
Chao ZhengState Key Laboratory of Breeding Base for Zhejiang Agricultural Products Quality and Safety, MOA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.
Jie ZhouState Key Laboratory of Breeding Base for Zhejiang Agricultural Products Quality and Safety, MOA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.
Bin ChenState Key Laboratory of Breeding Base for Zhejiang Agricultural Products Quality and Safety, MOA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.
Rumeng XuState Key Laboratory of Breeding Base for Zhejiang Agricultural Products Quality and Safety, MOA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.
Wenxia YuanState Key Laboratory of Breeding Base for Zhejiang Agricultural Products Quality and Safety, MOA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.
Ersong ZhengState Key Laboratory of Breeding Base for Zhejiang Agricultural Products Quality and Safety, MOA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.
Weifang LiangCollege of Plant Protection, Yunnan Agricultural University, Kunming, 650201, People's Republic of China.
Yong YangState Key Laboratory of Breeding Base for Zhejiang Agricultural Products Quality and Safety, MOA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.
Lijuan HeState Key Laboratory of Breeding Base for Zhejiang Agricultural Products Quality and Safety, MOA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.
Jianghua ShiState Key Laboratory of Breeding Base for Zhejiang Agricultural Products Quality and Safety, MOA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.
Chengqi YanInstitute of Biotechnology, Ningbo Academy of Agricultural Sciences, Ningbo, 315101, People's Republic of China.
Xuming WangState Key Laboratory of Breeding Base for Zhejiang Agricultural Products Quality and Safety, MOA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China. xmwang@zaas.ac.cn.
Jianping ChenCollege of Plant Protection, Yunnan Agricultural University, Kunming, 650201, People's Republic of China. jpchen2001@126.com.

Funding

the National Key Research and Development Program of China 2016YFD0100601-15the Zhejiang Provincial Natural Science Foundation of China LY16C140005the Zhejiang Provincial Natural Science Foundation of China LY17C130007the Zhejiang Provincial Natural Science Foundation of China LZ16C130002Zhejiang Fundamental Public Welfare Research Program LGN19C140008
6 · The paper itself

Abstract

DNA cloning is the basic step for different fields of life science, and many efforts have been made to simplify this procedure. In this study, we report two general purpose plasmids (pGP), pGP-XB2E and pGP-B2E, for rapid and cost-effective construct of basic clones. The BciVI and XcmI cleavage sites are designed in pGP-XB2E to test plasmid linearization efficiency. The plasmid has better linearization efficiency by using BciVI which could almost completely digest 2 μg plasmid in 10 min with only one-tenth the recommended enzyme concentration. In order to further optimize the pGP-XB2E, a new plasmid, pGP-B2E, which removed XcmI cleavage site was designed. This vector is highly efficient for cloning PCR products up to 1812 bp, and the number of colonies was about five times that of pGP-XB2E. In addition to TA cloning, blunt-end PCR products with T ended in the primer could be positively linked to the T-vector pGP-B2E without A-tailing treatment (TB cloning). Moreover, as an entry vector, pGP-B2E was also compatible for gateway recombination reaction without frameshift mutations. In general, this vector provides a universal, quick, and cost-efficient method for basic molecular cloning.

Indexed as

Cloning, MolecularGenetic VectorsCatalaseDNA LigasesGene ExpressionPlasmidsRecombinant Fusion ProteinsCatalaseDNA LigasesRecombinant Fusion ProteinsBlunt-end ligationGateway entry vectorHigh efficiencyLow costTA cloning

Identifiers

PMID31749084

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.