Evidence map›Paper›PMID 31748958›Full record

ArticleClinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico2020

The prognostic role of FZD6 in esophageal squamous cell carcinoma patients.

J Zhang, J-L Wang, C-Y Zhang, Y-F Ma, R Zhao, Y-Y Wang

Abstract read
PubMed Publisher
In one paragraph

Article in Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 18 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Roles of oncogenes in esophageal squamous cell carcinoma and their therapeutic potentials.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2023
    Review
  5. Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

J ZhangGraduate School, Ningxia Medical University, Yinchuan, 750004, Ningxia, China.
J-L WangGraduate School, Ningxia Medical University, Yinchuan, 750004, Ningxia, China.
C-Y ZhangDepartment of Radiation Oncology, General Hospital of Ningxia Medical University, No.804 Shengli Str., Yinchuan, 750004, Ningxia, China.
Y-F MaDepartment of Thoracic Surgery, General Hospital of Ningxia Medical University, Yinchuan, 750004, Ningxia, China.
R ZhaoDepartment of Radiation Oncology, General Hospital of Ningxia Medical University, No.804 Shengli Str., Yinchuan, 750004, Ningxia, China.
Y-Y WangDepartment of Radiation Oncology, General Hospital of Ningxia Medical University, No.804 Shengli Str., Yinchuan, 750004, Ningxia, China. fdwyy1981@hotmail.com.ORCID http://orcid.org/0000-0003-3626-8319
Ningxia Medical University · CNChinese People's Armed Police General Hospital · CNYinchuan First People's Hospital · CN

Funding

First-Class Discipline Construction Founded Project of NingXia Medical University and the School of Clinical Medicine NXYLXK2017A05National Natural Science Foundation of China 81502094
6 · The paper itself

Abstract

backgroundEsophageal squamous cell carcinoma (ESCC) is a kind of cancer with heterogeneous biological characteristics, which is affected by a complex network of gene interactions. Identification of molecular biomarkers paves the way for individualized therapy based on gene expression profiles, which can overcome the heterogeneity of ESCC.

methodsIn this study, GSE20347, GSE23400 and GSE45670 datasets were retrieved from Gene Expression Omnibus (GEO) database, and the overlapping differentially expressed genes (DEGs) in three datasets were screened. Then the overlapping DEGs function was annotated by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway-enrichment analysis. The prognostic value of the top five KEGG pathway-related genes were further validated in The Cancer Genome Atlas (TCGA) database. After extensive statistical analysis, four genes (CDC25B, CXCL8, FZD6 and MCM4) were identified as potential prognostic markers. Among the four candidate genes, the prognostic value of FZD6 in ESCC patients has not been evaluated. Therefore, we finally used immunohistochemistry method to evaluate the effect of FZD6 on the prognosis of patients with ESCC. Additionally, we detected the expression level of FZD6 in ESCC cell line and normal esophageal epithelial cell line, and observed the cell viability of ESCC cell line after FZD6 knockdown.

resultsThe results showed that the overexpression of FZD6 predicted poor overall survival (OS) (P = 0.005) and progression-free survival (PFS) (P = 0.004) in ESCC patients. COX regression analysis showed that N stage (P = 0.026) and FZD6 expression level (P = 0.001) were independent prognostic factors of OS for ESCC patients. Furthermore, compared with normal esophageal epithelial cell line, the up-regulation of FZD6 was detected in ESCC cell line. Knockdown of FZD6 could significantly inhibit the proliferation of ESCC cells (P < 0.001).

conclusionCDC25B, CXCL8, FZD6 and MCM4 were screened as candidate genes for prognosis assessment of patients with ESCC. The prognostic role of FZD6 in ESCC patients was confirmed in current study.

Indexed as

cdc25 PhosphatasesCell Line, TumorCell SurvivalEsophageal NeoplasmsEsophageal Squamous Cell CarcinomaFemaleFrizzled ReceptorsGene Knockdown TechniquesHumansImmunohistochemistryInterleukin-8In Vitro TechniquesMaleMiddle AgedMinichromosome Maintenance Complex Component 4Progression-Free SurvivalCDC25B protein, humancdc25 PhosphatasesCXCL8 protein, humanFrizzled ReceptorsFZD6 protein, humanInterleukin-8MCM4 protein, humanMinichromosome Maintenance Complex Component 4RNA, Small InterferingEsophageal squamous cell carcinomaFZD6Gene expression profilePrognosis

Identifiers

PMID31748958
OpenAlexW2991613149

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.