ArticleStem cell research & therapy2019
Autofluorescence of NADH is a new biomarker for sorting and characterizing cancer stem cells in human glioma.
Article in Stem cell research & therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Multimodal Biophysical Analysis of Morpho-Phenotypic and Morpho-Functional Dynamics in Normal and Cancer Spheroids within Engineered Microenvironments.Chemical & biomedical imaging · 2026Article
- A standardized single-tube 17-color spectral flow cytometry workflow for integrated immunophenotyping of human PBMCs and mixed co-culture systems.Frontiers in immunology · 2026Article
- Intracellular autofluorescence enables the isolation of viable, functional human muscle reserve cells with distinct Pax7 levels and stem cell states.Stem cell research & therapy · 2025Article
- Deep metabolic profiling of immune cells by spectral flow cytometry-A comprehensive validation approach.iScience · 2025Article
- Endothelial cell-derived SDF-1α elicits stemness traits of glioblastomaTheranostics · 2025Article
- A Redox-Shifted Fibroblast Subpopulation Emerges in the Fibrotic Lung.American journal of respiratory cell and molecular biology · 2024Article
- Metabolic profiling of single cells by exploiting NADH and FAD fluorescence via flow cytometry.Molecular metabolism · 2024Article
- Far-Red Fluorescent Proteins: Tools for Advancing In Vivo Imaging.Biosensors · 2024Review
- Role of glioma stem cells in promoting tumor chemo- and radioresistance: A systematic review of potential targeted treatments.World journal of stem cells · 2024Article
- The optimization and application of photodynamic diagnosis and autofluorescence imaging in tumor diagnosis and guided surgery: current status and future prospects.Frontiers in oncology · 2024Review
- NAD(P)H Activated Fluorescent Probe for Rapid Intraoperative Pathological Diagnosis and Tumor Histological Grading.Chemical & biomedical imaging · 2023Article
- Label-free optical imaging of cell function and collagen structure for cell-based therapies.Current opinion in biomedical engineering · 2023Article
- Distinguishing Healthy and Carcinoma Cell Cultures Using Fluorescence Spectra Decomposition with a Genetic-Algorithm-Based Code.Biosensors · 2023Article
- Single-cell imaging of α and β cell metabolic response to glucose in living human Langerhans islets.Communications biology · 2022Article
- Mitochondrial Matrix Protease ClpP Agonists Inhibit Cancer Stem Cell Function in Breast Cancer Cells by Disrupting Mitochondrial Homeostasis.Cancer research communications · 2022Article
- Review
- Near-infrared fluorescent probe based on rhodamine derivative for detection of NADH in live cells.Methods (San Diego, Calif.) · 2022Article
- Discrimination of cancerous from benign pigmented skin lesions based on multispectral autofluorescence lifetime imaging dermoscopy and machine learning.Journal of biomedical optics · 2022Article
- HIF1α/HIF2α induces glioma cell dedifferentiation into cancer stem cells through Sox2 under hypoxic conditions.Journal of Cancer · 2022Article
- Gene biomarker prediction in glioma by integrating scRNA-seq data and gene regulatory network.BMC medical genomics · 2021Article
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe existing cell surface markers used for sorting glioma stem cells (GSCs) have obvious limitations, such as vulnerability to the enzymatic digestion and time-consuming labeling procedure. Reduced nicotinamide adenine dinucleotide (NADH) as a cellular metabolite with property of autofluorescence has the potential to be used as a new biomarker for sorting GSCs.
methodsA method for sorting GSCs was established according to the properties of the autofluorescence of NADH. Then, the NADH
resultsNADH
conclusionOur findings demonstrate that intracellular autofluorescence of NADH is a non-labeling, sensitive maker for isolating GSCs, even for other CSCs.
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