ArticlePloS one2019
Detection of Schistosoma japonicum and Oncomelania hupensis quadrasi environmental DNA and its potential utility to schistosomiasis japonica surveillance in the Philippines.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.
- Detecting and identifying Schistosoma infections in snails and aquatic habitats: A systematic review.PLoS neglected tropical diseases · 2021Pooled it
- Development and field evaluation of a multiplex qPCR assay for environmental DNA detection of Schistosoma mekongi and its intermediate snail host Neotricula aperta in the Mekong River Basin.Infectious diseases of poverty · 2026Article
- Assessment of water prophylaxis and biosafety in finfish aquaculture - current and emerging technologies.Frontiers in microbiology · 2026Review
- Soil-based environmental DNA enables detection of Oncomelania hupensis quadrasi and Schistosoma japonicum microhabitats for schistosomiasis japonica surveillance and control in the Philippines.Infectious diseases of poverty · 2025Observational
- Benzalkonium Chloride Significantly Improves Environmental DNA Detection from Schistosomiasis Snail Vectors in Freshwater Samples.Tropical medicine and infectious disease · 2025Article
- Development of a SYBR Green-Based Real-Time PCR Assay to DetectTropical medicine and infectious disease · 2025Article
- Article
- Schistosomiasis in the Philippines: A Comprehensive Review of Epidemiology and Current Control.Tropical medicine and infectious disease · 2025Review
- Epidemiological Study on Humans, Animals and Snails for Schistosomiasis in Two Endemic Municipalities Nearing Elimination in Bohol, the Philippines.Acta parasitologica · 2025Article
- Improved environmental DNA detection sensitivity of Opisthorchis viverrini using a multi-marker assay.Parasitology research · 2024Article
- From Perpetual Wetness to Soil Chemistry: Enumerating Environmental and Physicochemical Factors FavoringTropical medicine and infectious disease · 2023Article
- Open-source environmental data as an alternative to snail surveys to assess schistosomiasis risk in areas approaching elimination.International journal of health geographics · 2023Article
- Assessment of schistosomiasis transmission in the River Nile at Greater Cairo using malacological surveys and cercariometry.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Environmental DNA in human and veterinary parasitology - Current applications and future prospects for monitoring and control.Food and waterborne parasitology · 2022Article
- Molecular Techniques as Alternatives of Diagnostic Tools in China as Schistosomiasis Moving towards Elimination.Pathogens (Basel, Switzerland) · 2022Review
- Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases.Clinical microbiology reviews · 2021Article
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, the prevalence and infection intensity of Schistosoma japonicum in endemic areas of the Philippines have significantly decreased due to yearly population-based treatment strategies, yet transmission rates remain high and uninterrupted. An important indicator of active disease transmission is the presence of Schistosoma japonicum and its snail intermediate host Oncomelania hupensis quadrasi in freshwater habitats. In this study, we sought to apply a species-specific real-time PCR (qPCR) assay for the detection of S. japonicum and O. hupensis quadrasi in freshwater samples using environmental DNA approach that can complement the commonly utilized malacological survey in determining potential transmission foci in order to have a more effective snail surveillance strategy for schistosomiasis japonica in endemic areas. The newly developed assay was specific to S. japonicum and O. hupensis quadrasi with no amplification detected against non-target trematode Fasciola spp. and snails such as Lymnaea spp., Pomacea canaliculata, and Melanoides spp. that typically co-exist in the same environment. The assay effectiveness was determined using 19 environmental water samples collected from Northern Samar (N = 5 sites), Leyte (N = 11 sites) and Compostela Valley (N = 3 sites) and compared to malacological survey for determining O. hupensis quadrasi snail colonies and snail crushing to visualize S. japonicum cercariae. TaqMan qPCR targeting a short fragment of the cytochrome c oxidase subunit 1 (cox1) gene was positive for S. japonicum in 9 sites, for O. hupensis quadrasi in 9 sites, and for both S. japonicum and O. hupensis quadrasi in 5 sampling sites. Moreover, it was able to detect O. hupensis quadrasi in 3 out of 12 sites found negative and 6 out of 7 sites found positive through malacological survey, and in 4 of the 5 snail sites positive for snails with cercariae. Overall, this method can complement malacological surveys for monitoring of schistosomes in endemic areas of the Philippines, especially those with high risk of human infection.
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