ArticleScientific reports2019
Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 91 citations in OpenAlex.
- Genome editing for vegetable crop improvement: Challenges and future prospects.Frontiers in genetics · 2022Pooled it
- Targeting a conserved functional motif in the PDS gene enables efficient CRISPR/Cas9 editing in banana.Scientific reports · 2026Article
- From recalcitrance to precision: a robust regeneration, transformation and targeted gene editing framework inFrontiers in genome editing · 2026Article
- CRISPR/Cas9-based gene-editing platform development by targeting genes encoding magnesium chelatase and phytoene desaturase in sugarbeet (Frontiers in genome editing · 2026Article
- Development ofFrontiers in genome editing · 2026Article
- CRISPR/Cas9-MediatedPlants (Basel, Switzerland) · 2025Article
- Ethylene promotes branch formation but inhibits tendril development in cucumber.Nature communications · 2025Article
- Multiplex CRISPR-Cas9 editing of chlorophyll biosynthesis genes in chickpea via protoplast and Agrobacterium-mediated transformation.Functional & integrative genomics · 2025Article
- Enhancing genetic transformation efficiency of melon (Cucumis melo L.) through an extended sucrose-removal co-culture.Plant cell reports · 2025Article
- Efficient CRISPR/Frontiers in plant science · 2025Article
- Unlocking the potential of 'Egusi' melon (Frontiers in plant science · 2025Review
- Article
- CRISPR/Cas9 based genome editing of Phytoene desaturase (PDS) gene in chilli pepper (Capsicum annuum L.).Journal, genetic engineering & biotechnology · 2024Article
- CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in pigeonpea and groundnut.Functional & integrative genomics · 2024Article
- CRISPR/Cas9 ribonucleoprotein mediated DNA-free genome editing in larch.Forestry research · 2024Article
- Parthenocarpy inPlants (Basel, Switzerland) · 2023Review
- Mutagenesis of the melon Prv gene by CRISPR/Cas9 breaks papaya ringspot virus resistance and generates an autoimmune allele with constitutive defense responses.Journal of experimental botany · 2023Article
- OptimisedGenes · 2023Article
- CRISPR/Cas9-Mediated Cytosine Base Editing Using an Improved Transformation Procedure in Melon (International journal of molecular sciences · 2023Article
- CRISPR/Cas9-mediated editing of phytoene desaturase (3 Biotech · 2023Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR/Cas9 system has been widely applied in many plant species to induce mutations in the genome for studying gene function and improving crops. However, to our knowledge, there is no report of CRISPR/Cas9-mediated genome editing in melon (Cucumis melo). In our study, phytoene desaturase gene of melon (CmPDS) was selected as target for the CRISPR/Cas9 system with two designed gRNAs, targeting exons 1 and 2. A construct (pHSE-CmPDS) carrying both gRNAs and the Cas9 protein was delivered by PEG-mediated transformation in protoplasts. Mutations were detected in protoplasts for both gRNAs. Subsequently, Agrobacterium-mediated transformation of cotyledonary explants was carried out, and fully albino and chimeric albino plants were successfully regenerated. A regeneration efficiency of 71% of transformed plants was achieved from cotyledonary explants, a 39% of genetic transformed plants were successful gene edited, and finally, a 42-45% of mutation rate was detected by Sanger analysis. In melon protoplasts and plants most mutations were substitutions (91%), followed by insertions (7%) and deletions (2%). We set up a CRISPR/Cas9-mediated genome editing protocol which is efficient and feasible in melon, generating multi-allelic mutations in both genomic target sites of the CmPDS gene showing an albino phenotype easily detectable after only few weeks after Agrobacterium-mediated transformation.
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