ArticleScientific reports2019
Molecular and genetic analysis of defensive responses of Brassica juncea - B. fruticulosa introgression lines to Sclerotinia infection.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Dissection of quantitative trait loci governing root system architecture under aluminium toxicity stress conditions in Brassica carinata-derived Brassica juncea introgression lines.BMC plant biology · 2026Article
- Multi-model genome-wide association analysis of agronomic traits in a Kabuli chickpea MAGIC-subset population (Frontiers in plant science · 2026Article
- Genetic and Epigenetic Mechanisms Underpinning Biotic Stress Resilience ofPlants (Basel, Switzerland) · 2025Review
- Dissection of QTLs conferring drought tolerance in B. carinata derived B. juncea introgression lines.BMC plant biology · 2023Article
- Genetic and molecular analysis of stem rot (Sclerotinia sclerotiorum) resistance in Brassica napus (canola type).Heliyon · 2023Article
- Utilization of crop wild relatives for biotic and abiotic stress management in Indian mustard [Frontiers in plant science · 2023Review
- Fungal effectors versus defense-related genes ofFrontiers in plant science · 2023Review
- Review
- Predicting Cloned Disease Resistance Gene Homologs (CDRHs) in Radish, Underutilised Oilseeds, and Wild Brassicaceae Species.Plants (Basel, Switzerland) · 2022Article
- The evolutionary and molecular features of the broad-host-range plant pathogen Sclerotinia sclerotiorum.Molecular plant pathology · 2022Review
- Article
- Sclerotinia sclerotiorum Thioredoxin1 (SsTrx1) is required for pathogenicity and oxidative stress tolerance.Molecular plant pathology · 2021Article
- Anchoring alien chromosome segment substitutions bearing gene(s) for resistance to mustard aphid in Brassica juncea-B. fruticulosa introgression lines and their possible disruption through gamma irradiation.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Article
- Understanding Host-Pathogen Interactions inPlants (Basel, Switzerland) · 2020Review
- Development of a Yellow-Seeded Stable Allohexaploid Brassica Through Inter-Generic Somatic Hybridization With a High Degree of Fertility and Resistance toFrontiers in plant science · 2020Article
- White Leaf Spot Caused byFrontiers in cellular and infection microbiology · 2020Review
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sclerotinia stem rot caused by Sclerotinia sclerotiorum is a major disease of crop brassicas, with inadequate variation for resistance in primary gene pools. We utilized a wild Brassicaceae species with excellent resistance against stem rot to develop a set of B. juncea - B. fruticulosa introgression lines (ILs). These were assessed for resistance using a highly reproducible stem inoculation technique against a virulent pathogen isolate. Over 40% of ILs showed higher levels of resistance. IL-43, IL-175, IL-215, IL-223 and IL-277 were most resistant ILs over three crop seasons. Sequence reads (21x) from the three most diverse ILs were then used to create B. juncea pseudomolecules, by replacing SNPs of reference B. juncea with those of re-sequenced ILs. Genotyping by sequencing (GBS) was also carried out for 88 ILs. Resultant sequence tags were then mapped on to the B. juncea pseudomolecules, and SNP genotypes prepared for each IL. Genome wide association studies helped to map resistance responses to stem rot. A total of 13 significant loci were identified on seven B. juncea chromosomes (A01, A03, A04, A05, A08, A09 and B05). Annotation of the genomic region around identified SNPs allowed identification of 20 candidate genes belonging to major disease resistance protein families, including TIR-NBS-LRR class, Chitinase, Malectin/receptor-like protein kinase, defensin-like (DEFL), desulfoglucosinolate sulfotransferase protein and lipoxygenase. A majority of the significant SNPs could be validated using whole genome sequences (21x) from five advanced generation lines being bred for Sclerotinia resistance as compared to three susceptible B. juncea germplasm lines. Our findings not only provide critical new understanding of the defensive pathway of B. fruticulosa resistance, but will also enable development of marker candidates for assisted transfer of introgressed resistant loci in to agronomically superior cultivars of crop Brassica.
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