ArticlePloS one2019
Hydrogen sulphide-induced hypometabolism in human-sized porcine kidneys.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Perfusate Composition and Duration ofTransplant international : official journal of the European Society for Organ Transplantation · 2022Pooled it
- UW supplementation with AP39 improves liver viability following static cold storage.Scientific reports · 2025Article
- Organ preservation: current limitations and optimization approaches.Frontiers in medicine · 2025Review
- Identification of pharmacological inducers of a reversible hypometabolic state for whole organ preservation.eLife · 2024Article
- Hydrogen Sulfide can Scavenge Free Radicals to Improve Spinal Cord Injury by Inhibiting the p38MAPK/mTOR/NF-κB Signaling Pathway.Neuromolecular medicine · 2024Review
- UW Supplementation with AP39 Improves Liver Viability Following Static Cold Storage.Research square · 2024Article
- Static Cold Storage with Mitochondria-Targeted Hydrogen Sulfide Donor Improves Renal Graft Function in an Ex Vivo Porcine Model of Controlled Donation-after-Cardiac-Death Kidney Transplantation.International journal of molecular sciences · 2023Article
- Loss of Endothelial Glycocalyx During Normothermic Machine Perfusion of Porcine Kidneys Irrespective of Pressure and Hematocrit.Transplantation direct · 2023Article
- HAntioxidants (Basel, Switzerland) · 2023Article
- Pushing the boundaries of innovation: the potential ofFrontiers in cardiovascular medicine · 2023Review
- Preoperative Function Assessment of Ex Vivo Kidneys with Supervised Machine Learning Based on Blood and Urine Markers Measured during Normothermic Machine Perfusion.Biomedicines · 2022Article
- Doxycycline Alters the Porcine Renal Proteome and Degradome during Hypothermic Machine Perfusion.Current issues in molecular biology · 2022Article
- Hydrogen Sulfide Actions in the Vasculature.Comprehensive Physiology · 2021Article
- Real-time visualization of renal microperfusion using laser speckle contrast imaging.Journal of biomedical optics · 2021Article
- Fighting Oxidative Stress with Sulfur: Hydrogen Sulfide in the Renal and Cardiovascular Systems.Antioxidants (Basel, Switzerland) · 2021Review
- Metformin Preconditioning and Postconditioning to Reduce Ischemia Reperfusion Injury in an Isolated Ex Vivo Rat and Porcine Kidney Normothermic Machine Perfusion Model.Clinical and translational science · 2021Article
- Hydrogen Sulfide and the Immune System.Advances in experimental medicine and biology · 2021Article
- Prolonged Organ Extraction Time Negatively Impacts Kidney Transplantation Outcome.Transplant international : official journal of the European Society for Organ Transplantation · 2021Article
- Renal Delivery of Pharmacologic Agents During Machine Perfusion to Prevent Ischaemia-Reperfusion Injury: From Murine Model to Clinical Trials.Frontiers in immunology · 2021Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSince the start of organ transplantation, hypothermia-forced hypometabolism has been the cornerstone in organ preservation. Cold preservation showed to protect against ischemia, although post-transplant injury still occurs and further improvement in preservation techniques is needed. We hypothesize that hydrogen sulphide can be used as such a new preservation method, by inducing a reversible hypometabolic state in human sized kidneys during normothermic machine perfusion.
methodsPorcine kidneys were connected to an ex-vivo isolated, oxygen supplemented, normothermic blood perfusion set-up. Experimental kidneys (n = 5) received a 85mg NaHS infusion of 100 ppm and were compared to controls (n = 5). As a reflection of the cellular metabolism, oxygen consumption, mitochondrial activity and tissue ATP levels were measured. Kidney function was assessed by creatinine clearance and fractional excretion of sodium. To rule out potential structural and functional deterioration, kidneys were studied for biochemical markers and histology.
resultsHydrogen sulphide strongly decreased oxygen consumption by 61%, which was associated with a marked decrease in mitochondrial activity/function, without directly affecting ATP levels. Renal biological markers, renal function and histology did not change after hydrogen sulphide treatment.
conclusionIn conclusion, we showed that hydrogen sulphide can induce a controllable hypometabolic state in a human sized organ, without damaging the organ itself and could thereby be a promising therapeutic alternative for cold preservation under normothermic conditions in renal transplantation.
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