ArticleArchives of insect biochemistry and physiology2020
Optimization of dietary RNA interference delivery to western flower thrips Frankliniella occidentalis and onion thrips Thrips tabaci.
Article in Archives of insect biochemistry and physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Efficacy of Select Gene Targets for the Management of Western Flower Thrips Through RNA Interference.Archives of insect biochemistry and physiology · 2026Article
- Basic Research on Whitefly Molecular Biology: A Foundation for Innovative Pest Management Strategies.Current issues in molecular biology · 2026Review
- Control of two sucking insect pests, a whitefly (Bemisia tabaci) and a thrips (Frankliniella occidentalis), infesting hot peppers by spraying LDH-formulated dsRNA.Pest management science · 2025Article
- Microinjection-enabled gene silencing in first instar larvae of western flower thrips, Frankliniella occidentalis, reveals vital genes for larval survival.Insect science · 2025Article
- Review
- Effects of Orally Delivered Double-Stranded RNA ofInsects · 2025Article
- Chloroplast Genome Engineering: A Plausible Approach to Combat Chili Thrips and Other Agronomic Insect Pests of Crops.Plants (Basel, Switzerland) · 2023Review
- Review
- Greenhouse test of spraying dsRNA to control the western flower thrips, Frankliniella occidentalis, infesting hot peppers.BMC biotechnology · 2023Article
- A narrow host-range and lack of persistence in two non-target insect species of a bacterial symbiont exploited to deliver insecticidal RNAi in Western Flower Thrips.Frontiers in insect science · 2023Article
- Effect of silencingFrontiers in microbiology · 2023Article
- Silencing of Thrips palmi UHRF1BP1 and PFAS Using Antisense Oligos Induces Mortality and Reduces Tospovirus Titer in Its VectorPathogens (Basel, Switzerland) · 2022Article
- Efficient control of western flower thrips by plastid-mediated RNA interference.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Bacterial Competition Influences the Ability of Symbiotic Bacteria to Colonize Western Flower Thrips.Frontiers in microbiology · 2022Article
- Current Status and Potential of RNA Interference for the Management of Tomato Spotted Wilt Virus and Thrips Vectors.Pathogens (Basel, Switzerland) · 2021Review
- Engineering a CulturableApplied and environmental microbiology · 2021Article
- Optimization of dietary RNA interference delivery to western flower thrips Frankliniella occidentalis and onion thrips Thrips tabaci.Archives of insect biochemistry and physiology · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
In insect reverse genetics, dietary delivery of interfering RNAs is a practical approach in nonmodel species, such as thrips, whose small size, and feeding behavior restricts the use of other delivery methods. In a laboratory context, an unsuitable diet could confound the interpretation of an RNA interference (RNAi) phenotype, however well-formulated artificial diets can minimize experimental variability, reduce the need for insect handling, and can further be used for roles, such as delivering double-strand RNA (dsRNA)-expressing recombinant bacteria. In this study, artificial diets for oral delivery of dsRNA were developed for two important pest thrips species, western flower thrips (Frankliniella occidentalis) and onion thrips (Thrips tabaci), with the goal of (a) stimulating feeding behavior, (b) supporting optimal growth rates of dsRNA-expressing symbiotic bacteria, and (c) nutritionally supporting the thrips for sufficient periods to observe RNAi phenotypes. The efficacy of artificial diets for ingesting "naked" dsRNA or dsRNA-expressing symbionts and dsRNA delivery via host plant uptake was evaluated. Compared with previously published diet formulations, new combinations based on tryptone, yeast, and soy were superior for enhancing feeding and longevity. However, simply adding "naked" dsRNA to an artificial diet was an unreliable form of RNAi delivery in our hands due to dsRNA degradation. Delivery via host plants was more successful, and the new diet formulation was suitable for symbiont-mediated dsRNA delivery, which we believe is the most convenient approach for large-scale knockdown experiments. This study, therefore, provides alternative methodologies for thrips rearing, dietary RNAi delivery, and insights into the challenges of performing dietary RNAi in nonmodel insects.
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