ArticleeLife2019
High-throughput microcircuit analysis of individual human brains through next-generation multineuron patch-clamp.
Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 90 citations in OpenAlex.
- Toward reliable machine learning models for neural circuit inference: A diagnostic study of CNNs on spike trains.PLoS computational biology · 2026Article
- DuBois-Reymond prize lecture 2024: Multi-neuron patch-clamp to uncover the neuronal and synaptic physiology in the human neocortex.Pflugers Archiv : European journal of physiology · 2026Article
- Transport-related effects on intrinsic and synaptic properties of human cortical neurons: A comparative study.The Journal of physiology · 2026Article
- Electrophysiological classification of human layer 2-3 pyramidal neurons reveals subtype-specific synaptic interactions.Nature neuroscience · 2026Article
- Deriving connectivity from spiking activity in detailed models of large-scale cortical microcircuits.iScience · 2026Article
- Enhanced Distal Signaling in Human Hippocampal Neurons despite Lower Intrinsic Excitability.Research square · 2025Article
- Principles of visual cortex excitatory microcircuit organization.Innovation (Cambridge (Mass.)) · 2025Article
- GABAergic synaptic components are largely preserved across human and mouse neuronal models.Frontiers in cellular neuroscience · 2025Article
- Synaptic transmission in supragranular layers of the human cortex - comparative review of structure, function, and plasticity.Frontiers in synaptic neuroscience · 2025Review
- Membrane potential states gate synaptic consolidation in human neocortical tissue.Nature communications · 2024Article
- Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections.eLife · 2024Article
- Article
- Large-Scale Mechanistic Models of Brain Circuits with Biophysically and Morphologically Detailed Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Review
- Patch-walking: Coordinated multi-pipette patch clamp for efficiently finding synaptic connections.bioRxiv : the preprint server for biology · 2024Article
- Volume electron microscopy analysis of synapses in primary regions of the human cerebral cortex.Cerebral cortex (New York, N.Y. : 1991) · 2024Article
- Human iPSC-Derived Neurons with Reliable Synapses and Large Presynaptic Action Potentials.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Structure and function of the hippocampal CA3 module.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Immediate reuse of patch-clamp pipettes after ultrasonic cleaning.Scientific reports · 2024Article
- Active Learning Exercises in Synaptic Physiology and Connectivity for the Neuroscience Lecture Hall, Laboratory Course, or Outreach Setting.Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2024Article
- 3D synaptic organization of layer III of the human anterior cingulate and temporopolar cortex.Cerebral cortex (New York, N.Y. : 1991) · 2023Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Comparing neuronal microcircuits across different brain regions, species and individuals can reveal common and divergent principles of network computation. Simultaneous patch-clamp recordings from multiple neurons offer the highest temporal and subthreshold resolution to analyse local synaptic connectivity. However, its establishment is technically complex and the experimental performance is limited by high failure rates, long experimental times and small sample sizes. We introduce an in vitro multipatch setup with an automated pipette pressure and cleaning system facilitating recordings of up to 10 neurons simultaneously and sequential patching of additional neurons. We present hardware and software solutions that increase the usability, speed and data throughput of multipatch experiments which allowed probing of 150 synaptic connections between 17 neurons in one human cortical slice and screening of over 600 connections in tissue from a single patient. This method will facilitate the systematic analysis of microcircuits and allow unprecedented assessment of inter-individual variability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.