Evidence map›Paper›PMID 31741893›Full record

ArticleChemosensors (Basel, Switzerland)2018

Using Fluorescence Intensity of Enhanced Green Fluorescent Protein to Quantify

Erin Wilson, Macduff Okuom, Nathan Kyes, Dylan Mayfield, Christina Wilson, Derek Sabatka, Jasmin Sandoval, Jared R Foote, Michael J Kangas, Andrea E Holmes and 1 more

Open access · goldAbstract read
In one paragraph

Article in Chemosensors (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
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  3. EngineeringJournal of materials chemistry. B · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Erin WilsonDepartment of Chemistry, Westminster College, New Wilmington, PA 16172, USA, wilsonee@westminster.edu.
Macduff OkuomDepartments of Chemistry, Doane University, Crete, NE 68333, USA.ORCID https://orcid.org/0000-0001-6004-8389
Nathan KyesDepartments of Chemistry, Doane University, Crete, NE 68333, USA.
Dylan MayfieldDepartments of Chemistry, Doane University, Crete, NE 68333, USA.
Christina WilsonDepartments of Chemistry, Doane University, Crete, NE 68333, USA.
Derek SabatkaDepartments of Chemistry, Doane University, Crete, NE 68333, USA.
Jasmin SandovalDepartments of Chemistry, Doane University, Crete, NE 68333, USA.
Jared R FooteDepartments of Chemistry, Doane University, Crete, NE 68333, USA.
Michael J KangasDepartments of Chemistry, Doane University, Crete, NE 68333, USA.ORCID https://orcid.org/0000-0001-6558-6928
Andrea E HolmesDepartments of Chemistry, Doane University, Crete, NE 68333, USA.
Arin L SutliefDepartments of Chemistry, Doane University, Crete, NE 68333, USA.
Doane University · USWestminster College - Pennsylvania · US

Funding

UNMC Structural Biology CoreP20GM103427 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI PAUL L SORGEN · 2012 to 2026
$59.2M
NIGMS NIH HHS P20 GM103427
6 · The paper itself

Abstract

A variety of direct and indirect methods have been used to quantify planktonic and biofilm bacterial cells. Direct counting methods to determine the total number of cells include plate counts, microscopic cell counts, Coulter cell counting, flow cytometry, and fluorescence microscopy. However, indirect methods are often used to supplement direct cell counting, as they are often more convenient, less time-consuming, and require less material, while providing a number that can be related to the direct cell count. Herein, an indirect method is presented that uses fluorescence emission intensity as a proxy marker for studying bacterial accumulation. A clinical strain of

Indexed as

bacterial quantificationbiofilmsenhanced green fluorescent proteinfluorescence spectroscopyfluorescent probePseudomonas aeruginosa

Identifiers

PMID31741893
PMCPMC6860918
OpenAlexW2801023067

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.