ArticleNature methods2020
Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution.
Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A connectome and analysis of the adulteLife · 2020Pooled it
- Electron microscopy-based three-dimensional subcellular imaging of plant male gametophyte.Journal of integrative plant biology · 2026Review
- Synaptic-resolution connectomics: towards large brains and connectomic screening.Nature reviews. Neuroscience · 2026Review
- Connectomics beyond electron microscopy.Nature methods · 2025Article
- Comparative prospects of imaging methods for whole-brain mammalian connectomics.Cell reports methods · 2025Review
- Neuronal wiring diagram of an adult brain.Nature · 2024Article
- Fast imaging of millimeter-scale areas with beam deflection transmission electron microscopy.Nature communications · 2024Article
- Scalable electron tomography for connectomics.bioRxiv : the preprint server for biology · 2024Article
- Array tomography: trails to discovery.Methods in microscopy · 2024Article
- GAUSS-EM, guided accumulation of ultrathin serial sections with a static magnetic field for volume electron microscopy.Cell reports methods · 2024Article
- Neuronal wiring diagram of an adult brain.bioRxiv : the preprint server for biology · 2023Article
- High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics.Nature methods · 2023Article
- Connectomics and the neural basis of behaviour.Current opinion in insect science · 2022Review
- Article
- How innovations in methodology offer new prospects for volume electron microscopy.Journal of microscopy · 2022Review
- Volume electron microscopy.Nature reviews. Methods primers · 2022Article
- Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy.Nature communications · 2022Article
- Article
- Single-Neuron Labeling in Fixed Tissue and Targeted Volume Electron Microscopy.Frontiers in neuroanatomy · 2022Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We demonstrate gas cluster ion beam scanning electron microscopy (SEM), in which wide-area ion milling is performed on a series of thick tissue sections. This three-dimensional electron microscopy technique acquires datasets with <10 nm isotropic resolution of each section, and these can then be stitched together to span the sectioned volume. Incorporating gas cluster ion beam SEM into existing single-beam and multibeam SEM workflows should be straightforward, increasing reliability while improving z resolution by a factor of three or more.
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Identifiers
31740820What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.