ArticleNature methods2020
Time-resolved serial femtosecond crystallography at the European XFEL.
Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed.
- Single-Ion Imaging Native Mass Spectrometry: Unraveling the Structural Features and Dissociation Energetics of Macromolecular Assemblies.Journal of the American Society for Mass Spectrometry · 2026Article
- Advances in Crystallography and Cryo-Electron Microscopy for the Study of Membrane Proteins.Advances in experimental medicine and biology · 2026Review
- Super Time-Resolved Tomography.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Denoising and iterative phase recovery reveal low-occupancy populations in protein crystals.Communications biology · 2025Article
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Standard Sample Preparation for Serial Femtosecond Crystallography.Biomolecules · 2025Review
- Ultrafast, remote-controlled protonation reaction enables structural changes in a phytochrome.Science advances · 2025Article
- Heavy-element damage seeding in proteins under XFEL illumination.Journal of synchrotron radiation · 2025Article
- Semi-automated modeling of reaction states in time-resolved serial femtosecond crystallography using molecular dynamics sampled conformations.Structural dynamics (Melville, N.Y.) · 2025Article
- Characterization of grating monochromator performance based on start-to-end FEL pulse propagation method.Journal of synchrotron radiation · 2025Article
- Superstability of micrometre jets surrounded by a polymeric shell.Journal of applied crystallography · 2025Article
- Observation of early events in the photoactivation of Myxobacterial phytochrome using time-resolved serial femtosecond crystallography.Communications chemistry · 2025Article
- A tunable and versatile chemogenetic near-infrared fluorescent reporter.Nature communications · 2025Article
- SFCalculator: connecting deep generative models and crystallography.bioRxiv : the preprint server for biology · 2025Article
- Filling data analysis gaps in time-resolved crystallography by machine learning.Structural dynamics (Melville, N.Y.) · 2025Article
- Exploiting fourth-generation synchrotron radiation for enzyme and photoreceptor characterization.IUCrJ · 2025Article
- Structural effects of high laser power densities on an early bacteriorhodopsin photocycle intermediate.Nature communications · 2024Article
- Small-rotative fixed-target serial synchrotron crystallography (SR-FT-SSX) for molecular crystals.Communications chemistry · 2024Article
- ResiDEM: Analytical Tool for Isomorphous Difference Electron Density Maps Utilizing Dynamic Residue Identification via Density Clustering.Journal of chemical information and modeling · 2024Article
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24 more citing papers are in PubMed but not listed here.
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48 authors.
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Abstract
The European XFEL (EuXFEL) is a 3.4-km long X-ray source, which produces femtosecond, ultrabrilliant and spatially coherent X-ray pulses at megahertz (MHz) repetition rates. This X-ray source has been designed to enable the observation of ultrafast processes with near-atomic spatial resolution. Time-resolved crystallographic investigations on biological macromolecules belong to an important class of experiments that explore fundamental and functional structural displacements in these molecules. Due to the unusual MHz X-ray pulse structure at the EuXFEL, these experiments are challenging. Here, we demonstrate how a biological reaction can be followed on ultrafast timescales at the EuXFEL. We investigate the picosecond time range in the photocycle of photoactive yellow protein (PYP) with MHz X-ray pulse rates. We show that difference electron density maps of excellent quality can be obtained. The results connect the previously explored femtosecond PYP dynamics to timescales accessible at synchrotrons. This opens the door to a wide range of time-resolved studies at the EuXFEL.
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