Evidence map›Paper›PMID 31740287›Full record

ArticleReproductive toxicology (Elmsford, N.Y.)2020

Anchoring a dynamic in vitro model of human neuronal differentiation to key processes of early brain development in vivo.

Susanna H Wegner, Julie Juyoung Park, Tomomi Workman, Sanne A B Hermsen, Jim Wallace, Ian B Stanaway, Hee Yeon Kim, William C Griffith, Sungwoo Hong, Elaine M Faustman

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive toxicology (Elmsford, N.Y.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Temporal Transcriptional Regulation of Human Neuronal Differentiation via Forward Programming.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  2. Pluripotent stem cells for target organ developmental toxicity testing.Toxicological sciences : an official journal of the Society of Toxicology · 2024
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Susanna H WegnerInstitute for Risk Analysis and Risk Communication, Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, WA, United States.
Julie Juyoung ParkInstitute for Risk Analysis and Risk Communication, Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, WA, United States.
Tomomi WorkmanInstitute for Risk Analysis and Risk Communication, Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, WA, United States.
Sanne A B HermsenInstitute for Risk Analysis and Risk Communication, Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, WA, United States.
Jim WallaceInstitute for Risk Analysis and Risk Communication, Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, WA, United States.
Ian B StanawayInstitute for Risk Analysis and Risk Communication, Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, WA, United States.
Hee Yeon KimInstitute for Risk Analysis and Risk Communication, Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, WA, United States.
William C GriffithInstitute for Risk Analysis and Risk Communication, Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, WA, United States.
Sungwoo HongInstitute for Risk Analysis and Risk Communication, Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, WA, United States.
Elaine M FaustmanInstitute for Risk Analysis and Risk Communication, Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, WA, United States. Electronic address: faustman@u.washington.edu.
University of Washington · US

Funding

XENOBIOTIC BIOTRANSFORMATION AND DISPOSITIONP30ES007033 · NIEHS · UNIVERSITY OF WASHINGTON · PI Sheela Sathyanarayana · 1995 to 2026
$42.5M
Environmetal Pathology/Toxicology Training ProgramT32ES007032 · NIEHS · UNIVERSITY OF WASHINGTON · PI FAUSTMAN, ELAINE M, ROSENFELD, MICHAEL E · 1985 to 2023
$10.7M
Biostatistics, Epidemiologic & Bioinformatic Training in Environmental HealthT32ES015459 · NIEHS · UNIVERSITY OF WASHINGTON · PI June T. Spector · 2009 to 2026
$10.6M
Mechanisms of Brain Dysmorphology in MN1 C-Terminal Truncation Syndrome, a Novel Intellectual Developmental Disability Disorder P50HD103524 · NICHD · UNIVERSITY OF WASHINGTON · PI Sandra E Juul · 2020 to 2026
$9.9M
STUDY OF CELLULAR &MOLECULAR ASPECTS OF PESTICIDE NEURODEVELOPMENTAL TOXICITYP01ES009601 · NIEHS · UNIVERSITY OF WASHINGTON · PI THOMPSON, ENGELBERTA · 1999 to 2013
$8.7M
FDA HHS U01 FD004242NICHD NIH HHS P50 HD103524NIEHS NIH HHS P01 ES009601NIEHS NIH HHS P30 ES007033NIEHS NIH HHS T32 ES007032NIEHS NIH HHS T32 ES015459
6 · The paper itself

Abstract

We characterize temporal pathway dynamics of differentiation in an in vitro neurotoxicity model with the aim of informing design and interpretation of toxicological assays. Human neural progenitor cells (hNPCs) were cultured in differentiation conditions up to 21 days. Genes significantly changed through time were identified and grouped according to temporal dynamics. Quantitative pathway analysis identified gene ontology (GO) terms enriched among significantly changed genes and provided a temporal roadmap of pathway trends in vitro. Gene expression in hNPCs was compared with publicly available gene expression data from developing human brain tissue in vivo. Quantitative pathway analysis of significantly changed genes and targeted analysis of specific pathways of interest identified concordance between in vivo and in vitro expression associated with proliferation, migration, differentiation, synapse formation, and neurotransmission. Our analysis anchors gene expression patterns in vitro to sensitive windows of in vivo development, helping to define appropriate applications of the model.

Indexed as

Models, BiologicalNeurogenesisBrainCells, CulturedCell SurvivalFemaleHumansNeural Stem CellsNeurotoxicity SyndromesTranscriptomeBrain developmentDevelopmental pathway dynamicsNeuronal differentiation

Identifiers

PMID31740287
PMCPMC6980388
OpenAlexW2985242335

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.