Evidence map›Paper›PMID 31739786›Full record

ArticleMicrobial cell factories2019

Substantially improving the enantioconvergence of PvEH1, a Phaseolus vulgaris epoxide hydrolase, towards m-chlorostyrene oxide by laboratory evolution.

Xun-Cheng Zong, Chuang Li, Yao-Hui Xu, Die Hu, Bo-Chun Hu, Jia Zang, Min-Chen Wu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Epoxide Hydrolases: Multipotential Biocatalysts.International journal of molecular sciences · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Xun-Cheng ZongKey Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Chuang LiKey Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Yao-Hui XuThe Affiliated Wuxi Matemity and Child Health Care Hospital of Nanjing Medical University, Wuxi, 214002, China.
Die HuWuxi School of Medicine, Jiangnan University, Wuxi, 214122, China.
Bo-Chun HuKey Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Jia ZangThe Affiliated Wuxi Matemity and Child Health Care Hospital of Nanjing Medical University, Wuxi, 214002, China. zangj1976@sina.com.
Min-Chen WuWuxi School of Medicine, Jiangnan University, Wuxi, 214122, China. biowmc@126.com.
Jiangnan University · CNNanjing Maternity and Child Health Care Hospital · CNNanjing Medical University · CN

Funding

National Natural Science Foundation of China No. 21676117Postgraduate Research and Practice Innovation Program of Jiangsu Provence SJX17_0503
6 · The paper itself

Abstract

backgroundEpoxide hydrolase can regioselectively catalyze the oxirane ring-opening hydrolysis of rac-epoxides producing the corresponding chiral diols. In our laboratory, a gene named pveh1 encoding an EH from Phaseolus vulgaris was cloned. Although the directed modification of PvEH1 was carried out, the mutant PvEH1

resultsPvEH1 and PvEH1

conclusionsIn summary, the enantioconvergence of PvEH1

Indexed as

Directed Molecular EvolutionEpoxide HydrolasesEscherichia coliGenes, PlantModels, MolecularMutagenesis, Site-DirectedPhaseolusTransformation, BacterialEpoxide HydrolasesEnantioconvergenceEpoxide hydrolaseLaboratory evolutionm-Chlorophenyl-1,2-ethanediolm-Chlorostyrene oxideSubstrate-binding pocket

Identifiers

PMID31739786
PMCPMC6859628
OpenAlexW2986168137

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.