ArticleMicrobial cell factories2019
Synthetic control devices for gene regulation in Penicillium chrysogenum.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
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- Establishment of the Genetic Transformation System ofJournal of fungi (Basel, Switzerland) · 2026Article
- CRISPR/Cas systems in fungal biotechnology: advancing high-value metabolite synthesis for industrial and food security applications.Archives of microbiology · 2026Review
- Development of a CRISPR/Cas9 RNP-mediated genetic engineering system in Paecilomyces variotii.Journal of microbiology (Seoul, Korea) · 2025Article
- Tracking the infection dynamics ofFrontiers in plant science · 2025Article
- Heterologous Naringenin Production in the Filamentous FungusJournal of agricultural and food chemistry · 2023Article
- CRISPR/Cas9-Mediated Disruption of theJournal of fungi (Basel, Switzerland) · 2023Article
- Review
- Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi.Frontiers in bioengineering and biotechnology · 2022Review
- Ten decadal advances in fungal biology leading towards human well-being.Fungal diversity · 2022Review
- The Confusion in Renaming Species: Penicillium chrysogenum and Penicillium rubens.Microbiology resource announcements · 2021Article
- Modular Synthetic Biology Toolkit for Filamentous Fungi.ACS synthetic biology · 2021Article
- Development of an Haa1-based biosensor for acetic acid sensing in Saccharomyces cerevisiae.FEMS yeast research · 2021Article
- Identification of a conserved N-terminal domain in the first module of ACV synthetases.MicrobiologyOpen · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
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Abstract
backgroundOrthogonal, synthetic control devices were developed for Penicillium chrysogenum, a model filamentous fungus and industrially relevant cell factory. In the synthetic transcription factor, the QF DNA-binding domain of the transcription factor of the quinic acid gene cluster of Neurospora crassa is fused to the VP16 activation domain. This synthetic transcription factor controls the expression of genes under a synthetic promoter containing quinic acid upstream activating sequence (QUAS) elements, where it binds. A gene cluster may demand an expression tuned individually for each gene, which is a great advantage provided by this system.
resultsThe control devices were characterized with respect to three of their main components: expression of the synthetic transcription factors, upstream activating sequences, and the affinity of the DNA binding domain of the transcription factor to the upstream activating domain. This resulted in synthetic expression devices, with an expression ranging from hardly detectable to a level similar to that of highest expressed native genes. The versatility of the control device was demonstrated by fluorescent reporters and its application was confirmed by synthetically controlling the production of penicillin.
conclusionsThe characterization of the control devices in microbioreactors, proved to give excellent indications for how the devices function in production strains and conditions. We anticipate that these well-characterized and robustly performing control devices can be widely applied for the production of secondary metabolites and other compounds in filamentous fungi.
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