ArticleEpigenetics2020
MBD-seq - realities of a misunderstood method for high-quality methylome-wide association studies.
Article in Epigenetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A methylation study implicates the rewiring of brain neural circuits during puberty in the emergence of sex differences in depression symptoms.Journal of child psychology and psychiatry, and allied disciplines · 2022Pooled it
- Methylated circulating tumor DNA: technical challenges and clinical applications in non-small cell lung cancer patients-a narrative review.Translational lung cancer research · 2026Review
- Methods for Investigating the Sperm Epigenome.Advances in experimental medicine and biology · 2026Review
- Insights from a methylome-wide association study of antidepressant exposure.Nature communications · 2025Article
- Epigenetic study of the long-term effects of gulf War illness.Frontiers in genetics · 2025Article
- Mining nucleic acid "omics" to boost liquid biopsy in cancer.Cell reports. Medicine · 2024Review
- Deciphering DNA Methylation in Gestational Diabetes Mellitus: Epigenetic Regulation and Potential Clinical Applications.International journal of molecular sciences · 2024Review
- The Impact of Childhood Mental Health and Substance Use on Methylation Aging Into Adulthood.Journal of the American Academy of Child and Adolescent Psychiatry · 2024Article
- Translation of Epigenetics in Cell-Free DNA Liquid Biopsy Technology and Precision Oncology.Current issues in molecular biology · 2024Review
- Investigating neonatal health risk variables through cell-type specific methylome-wide association studies.Clinical epigenetics · 2024Article
- Cell-type specific methylation changes in the newborn child associated to obstetric pain relief.PloS one · 2024Article
- Article
- Methylome-wide association study of anxiety disorders.Molecular psychiatry · 2023Article
- Genes implicated by a methylome-wide schizophrenia study in neonatal blood show differential expression in adult brain samples.Molecular psychiatry · 2023Article
- Epigenetic analysis in a murine genetic model of Gulf War illness.Frontiers in toxicology · 2023Article
- Genome wide exploration of the methylome in aggressive B-cell lymphoma in Golden Retrievers reveals a conserved hypermethylome.Epigenetics · 2022Article
- Early adversities accelerate epigenetic aging into adulthood: a 10-year, within-subject analysis.Journal of child psychology and psychiatry, and allied disciplines · 2022Article
- DNA methylation signatures of childhood trauma predict psychiatric disorders and other adverse outcomes 17 years after exposure.Molecular psychiatry · 2022Article
- Dual methylation and hydroxymethylation study of alcohol use disorder.Addiction biology · 2022Article
- DNA methylation of the KLK8 gene in depression symptomatology.Clinical epigenetics · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The majority of methylome-wide association studies (MWAS) have been performed using commercially available array-based technologies such as the Infinium Human Methylation 450K and the Infinium MethylationEPIC arrays (Illumina). While these arrays offer a convenient and relatively robust assessment of the probed sites they only allow interrogation of 2-4% of all CpG sites in the human genome. Methyl-binding domain sequencing (MBD-seq) is an alternative approach for MWAS that provides near-complete coverage of the methylome at similar costs as the array-based technologies. However, despite publication of multiple positive evaluations, the use of MBD-seq for MWAS is often fiercely criticized. Here we discuss key features of the method and debunk misconceptions using empirical data. We conclude that MBD-seq represents an excellent approach for large-scale MWAS and that increased utilization is likely to result in more discoveries, advance biological knowledge, and expedite the clinical translation of methylome-wide research findings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.