ArticleFrontiers in bioengineering and biotechnology2019
Multi-Layer Controls of Cas9 Activity Coupled With ATP Synthase Over-Expression for Efficient Genome Editing in
Article in Frontiers in bioengineering and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- CRISPR/Cas9-Mediated Polyketide Synthase Replacement for High-Yield Biosynthesis and Biological Activity of Milbemycin D.Biology · 2026Article
- A CRISPR-Cas9 system for knock-out and knock-in of high molecular weight DNA enables module-swapping of the pikromycin synthase in its native host.Microbial cell factories · 2025Article
- Highly efficient CRISPR-Cas9 base editing inApplied and environmental microbiology · 2025Article
- Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production.Nature communications · 2025Article
- CRISPR/Cas genome editing in plants: mechanisms, applications, and overcoming bottlenecks.Functional & integrative genomics · 2024Review
- Construction of a bacteriophage-derived recombinase system in Bacillus licheniformis for gene deletion.AMB Express · 2023Article
- Two strategies to improve the supply of PKS extender units for ansamitocin P-3 biosynthesis by CRISPR-Cas9.Bioresources and bioprocessing · 2022Article
- Redirection of acyl donor metabolic flux for lipopeptide A40926B0 biosynthesis.Microbial biotechnology · 2022Article
- Engineering Modular Polyketide Biosynthesis in Streptomyces Using CRISPR/Cas: A Practical Guide.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A Cas12a-based gene editing system for Phytophthora infestans reveals monoallelic expression of an elicitor.Molecular plant pathology · 2021Article
- Regulation of Protein Post-Translational Modifications on Metabolism of Actinomycetes.Biomolecules · 2020Review
- Challenges and Advances in Genome Editing Technologies inBiomolecules · 2020Review
- Fine-tuning the regulation of Cas9 expression levels for efficient CRISPR-Cas9 mediated recombination in Streptomyces.Journal of industrial microbiology & biotechnology · 2020Article
- CRISPR/Cas13d-Mediated Microbial RNA Knockdown.Frontiers in bioengineering and biotechnology · 2020Article
- Modern Approaches to the Genome Editing of Antibiotic Biosynthetic Clusters in Actinomycetes.Acta naturaeArticle
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient genome editing is a prerequisite of genetic engineering in synthetic biology, which has been recently achieved by the powerful CRISPR/Cas9 system. However, the toxicity of Cas9, due to its abundant intracellular expression, has impeded its extensive applications. Here we constructed a genetic cassette with triple controls of Cas9 activities at transcriptional, translational and protein levels, together with over-expression of the ATP synthase β-subunit AtpD, for the efficient genome editing in
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