ArticleJournal of analytical methods in chemistry2019
Design and Synthesis of Ag Nanocluster Molecular Beacon for Adenosine Triphosphate Detection.
Article in Journal of analytical methods in chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Breaking the silence: a cross-sectional study of psychological capital, job embeddedness, and silence behavior in operating room nurses.Frontiers in medicine · 2026Article
- Multi-Factors Cooperatively Actuated Photonic Hydrogel Aptasensors for Facile, Label-Free and Colorimetric Detection of Lysozyme.Biosensors · 2022Article
- A molecular beacon-like Ag nanocluster fluorescence probe for nucleic acid detection.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2022Article
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Authors and funding
8 authors.
Funding
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Abstract
This study presents a fluorescence method for detecting adenosine triphosphate (ATP) based on a label-free Ag nanocluster molecular beacon (MB) with high sensitivity. The sensor contains a hairpin-shaped MB, two short single-stranded DNA strands, and T4 DNA ligase. The MB consists of three parts, which are the template DNA sequence for synthesizing Ag nanoclusters at the 5' end, the middle DNA with a hairpin-shaped structure, and the guanine base-rich DNA sequence at the 3' end. The sensor exhibits high fluorescence intensity in the absence of ATP. However, when the probe is used for ATP detection, the two short DNA sequences in the sensor would form a long sequence by enzymatic ligation reaction; this long sequence opens the hairpin-shaped structure of the MB and decreases the fluorescence of the system. Under optimal analytical conditions, a clear linear relationship is observed between ATP concentration and fluorescence intensity in the range of 0.1-10
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Registered trials
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