ArticleStem cells international2019
Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation.
Article in Stem cells international, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
11 citing papers in PubMed.
- Correction to "Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation".Stem cells international · 2026Article
- Structurally and mechanically tuned macroporous hydrogels for scalable mesenchymal stem cell-extracellular matrix spheroid production.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Three-Dimensional Spheroid Culture of Human Mesenchymal Stem Cells: Offering Therapeutic Advantages and In Vitro Glimpses of the In Vivo State.Stem cells translational medicine · 2023Review
- Light-controlled scaffold- and serum-free hard palatal-derived mesenchymal stem cell aggregates for bone regeneration.Bioengineering & translational medicine · 2023Article
- Engineering plants with carbon nanotubes: a sustainable agriculture approach.Journal of nanobiotechnology · 2022Review
- Three-dimensional cultured mesenchymal stem cells enhance repair of ischemic stroke through inhibition of microglia.Stem cell research & therapy · 2021Article
- Generation of Therapeutically Potent Spheroids from Human Endometrial Mesenchymal Stem/Stromal Cells.Journal of personalized medicine · 2021Article
- Proteomics reveals the function reverse of MPSSS-treated prostate cancer-associated fibroblasts to suppress PC-3 cell viability via the FoxO pathway.Cancer medicine · 2021Article
- Cancer stem cells as a therapeutic target in 3D tumor models of human chondrosarcoma: An encouraging future for proline rich polypeptide‑1.Molecular medicine reports · 2020Article
- Xeno-Free Spheroids of Human Gingiva-Derived Progenitor Cells for Bone Tissue Engineering.Frontiers in bioengineering and biotechnology · 2020Article
- Retraction: Tumor Targeting by Conditioned Medium Derived from Human Amniotic Membrane: New Insight in Breast Cancer Therapy.Technology in cancer research & treatmentArticle
Corrections and comments
- Erratum issued
Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human mesenchymal stem cells (hMSCs) are widely used in clinical research because of their multipotential, immunomodulatory, and reparative properties. Previous studies determined that hMSC spheroids from a three-dimensional (3D) culture possess higher therapeutic efficacy than conventional hMSCs from a monolayer (2D) culture. To date, various 3D culture methods have been developed to form hMSC spheroids but most of them used culture medium containing fetal bovine serum (FBS), which is not suitable for further clinical use. Here, we demonstrate that dissociated single MSCs seeded in induced pluripotent stem medium (MiPS) adhere loosely to the dish and spontaneously migrate to form spheroids during day 3 to day 6. Through component deletion screening and complementation experiments, the knockout serum replacement (KSR) was identified as necessary and sufficient for hMSC spheroid formation. Transcriptome analysis showed that the overall expression profiles were highly similar between 2D culture with FBS and KSR-derived spheroids. Interestingly, genes related to inflammatory response, immune response, and angiogenesis were upregulated in spheroids at day 6 and qPCR results further validated the increased expression level of related genes, including
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