ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2020
Phosphotungstic acid-enhanced microCT: Optimized protocols for embryonic and early postnatal mice.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03025763 (Craniosynostosis Network), which is not on this map. Cited by 53 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Craniosynostosis Network
Who cites it
53 citing papers in PubMed.
- Pulmonary development in Squamata: Insights from embryonic studies using micro-CT.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Protocol for Post-Mortem Micro-CT Imaging of Coronary Arteries in Low-Mass Neonatal Puppy Hearts Using Barium-Based Contrast.Animals : an open access journal from MDPI · 2026Article
- A novel mouse model of rare neurodevelopmental disorder, TBCK Syndrome.bioRxiv : the preprint server for biology · 2026Article
- 3D vascular quantitation with application to computational modeling: a pre-clinical light sheet microscopy, high resolution ultrasound, nano-computed tomography comparison study.bioRxiv : the preprint server for biology · 2026Article
- Synchrotron radiation micro-computed tomography of the small-spotted catshark embryonic development (Chondrichthyes: Scyliorhinus canicula).GigaScience · 2026Article
- Luminescent mouse model of endometriosis: three-dimensional morphology of lesions and cytokine profiles.Experimental animals · 2025Article
- Embryonic cranial cartilage defects in the Fgfr3Anatomical record (Hoboken, N.J. : 2007) · 2025Article
- Differential performance of aqueous- and ethylic-Lugol's iodine stain to visualize anatomy in μCT-scanned vertebrates.Journal of anatomy · 2025Article
- Unveiling Another Dimension: Advanced Visualization of Cancer Invasion and Metastasis via Micro-CT Imaging.Cancers · 2025Article
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- In situ isotropic 3D imaging of vasculature perfusion specimens using x-ray microscopic dual-energy CT.Journal of microscopy · 2025Article
- Universal conditional networks (UniCoN) for multi-age embryonic cartilage segmentation with Sparsely annotated data.Scientific reports · 2025Article
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- Therapy to teratology: chronic paternal antioxidant supplementation alters offspring placental architecture and craniofacial morphogenesis in a mouse model.Frontiers in cell and developmental biology · 2025Article
- Phosphotungstic acid-enhanced micro-computed tomography and RNA sequencing provide a new perspective on temporomandibular joint arthritis induced by complete Freund's adjuvant and collagen-induced arthritis in rat models.Journal of dental sciences · 2025Article
- The SmARTR pipeline: A modular workflow for the cinematic rendering of 3D scientific imaging data.iScience · 2024Article
- Micro-CT study of human laryngeal structures using phosphotungstic agent staining.Surgical and radiologic anatomy : SRA · 2024Article
- Analysis of the Actions of RARγ Agonists on Growing Osteochondromas in a Mouse Model.International journal of molecular sciences · 2024Article
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7 authors.
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Abstract
backgroundGiven the need for descriptive and increasingly mechanistic morphological analyses, contrast-enhanced microcomputed tomography (microCT) represents perhaps the best method for visualizing 3D biological soft tissues in situ. Although staining protocols using phosphotungstic acid (PTA) have been published with beautiful visualizations of soft tissue structures, these protocols are often aimed at highly specific research questions and are applicable to a limited set of model organisms, specimen ages, or tissue types. We provide detailed protocols for micro-level visualization of soft tissue structures in mice at several embryonic and early postnatal ages using PTA-enhanced microCT.
resultsOur protocols produce microCT scans that enable visualization and quantitative analyses of whole organisms, individual tissues, and organ systems while preserving 3D morphology and relationships with surrounding structures, with minimal soft tissue shrinkage. Of particular note, both internal and external features of the murine heart, lungs, and liver, as well as embryonic cartilage, are captured at high resolution.
conclusionThese protocols have broad applicability to mouse models for a variety of diseases and conditions. Minor experimentation in the staining duration can expand this protocol to additional age groups, permitting ontogenetic studies of internal organs and soft tissue structures within their 3D in situ position.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.