ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2020
Inhibition monitoring in veterinary molecular testing.
Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Development and evaluation of a single-tube RT-ERA-CRISPR/Cas12a assay for simultaneous detection of enterovirus G and recombinant strains with papain-like cysteine protease gene insertion.Veterinary research communications · 2026Article
- Matrix extension to bovine feces and evaluation of semi-automated DNA extraction methods for the detection ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2026Article
- Evaluation of IndiMix JOE with Intype IC-RNA as an Alternative to AgPath-ID for Influenza A Virus Detection in Avian and Bovine Samples.Pathogens (Basel, Switzerland) · 2026Article
- Development of a new TaqMan real-time PCR for detecting Psittacine adenovirus 1 (PsAdV-1).BMC veterinary research · 2026Article
- A One Health Approach to Hepatitis E Virus in Venezuela: Low Seroprevalence in Humans and First Genomic Evidence of Hepatitis E Virus Genotype 3 in a Domestic Swine.Microorganisms · 2026Article
- Detection of the BK virus in urban wastewater inlets into the Karun River.Iranian journal of microbiology · 2025Article
- Optimizing Nucleic Acid Extraction from Extended Bovine Semen for Endemic and High-Consequence Pathogens.Animals : an open access journal from MDPI · 2025Article
- Prevalence of Onchocerca lupi in shelter dogs from an endemic region of the Southwestern USA.Parasites & vectors · 2025Article
- Detection and quantification ofThe veterinary quarterly · 2024Article
- An Assessment of Diagnostic Assays and Sample Types in the Detection of an Attenuated Genotype 5 African Swine Fever Virus in European Pigs over a 3-Month Period.Pathogens (Basel, Switzerland) · 2022Article
- Porcine reproductive and respiratory syndrome virus whole-genome sequencing efficacy with field clinical samples using a poly(A)-tail viral genome purification method.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- Infectious disease surveillance of apparently healthy horses at a multi-day show using a novel nanoscale real-time PCR panel.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- Focus issue on best practices for development, validation, and use of PCR assays.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Article
- Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Article
- Suggested guidelines for validation of real-time PCR assays in veterinary diagnostic laboratories.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many of the sample matrices typically used for veterinary molecular testing contain inhibitory factors that can potentially reduce analytic sensitivity or produce false-negative results by masking the signal produced by the nucleic acid target. Inclusion of internal controls in PCR-based assays is a valuable strategy not only for monitoring for PCR inhibitors, but also for monitoring nucleic acid extraction efficiency, and for identifying technology errors that may interfere with the ability of an assay to detect the intended target. The Laboratory Technology Committee of the American Association of Veterinary Laboratory Diagnosticians reviewed the different types of internal controls related to monitoring inhibition of PCR-based assays, and provides information here to encourage veterinary diagnostic laboratories to incorporate PCR internal control strategies as a routine quality management component of their molecular testing.
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