ArticleMikrochimica acta2019
A hybridization chain reaction based assay for fluorometric determination of exosomes using magnetic nanoparticles and both aptamers and antibody as recognition elements.
Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- How is Nanoarchitectonics Shaping Extracellular Vesicle Research?Advanced healthcare materials · 2026Review
- Nanomaterial-Assisted Physical Mass Loading and Signal Amplification Strategies for Exosome Isolation and Sensing in Liquid Biopsy: A Review.Biosensors · 2026Review
- Biogenesis, Isolation, and Detection of Exosomes and Their Potential in Therapeutics and Diagnostics.Biosensors · 2023Review
- Nanomaterials for Molecular Detection and Analysis of Extracellular Vesicles.Nanomaterials (Basel, Switzerland) · 2023Review
- Recent advances in nanotechnology-enabled biosensors for detection of exosomes as new cancer liquid biopsy.Experimental biology and medicine (Maywood, N.J.) · 2022Review
- Harnessing the Therapeutic Potential of Extracellular Vesicles for Biomedical Applications Using Multifunctional Magnetic Nanomaterials.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Aptasensors for Cancerous Exosome Detection.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Exosomes as Powerful Engines in Cancer: Isolation, Characterization and Detection Techniques.Biosensors · 2021Review
- Progress in Nanomaterials-Based Optical and Electrochemical Methods for the Assays of Exosomes.International journal of nanomedicine · 2021Review
- Immunoassay-type biosensor based on magnetic nanoparticle capture and the fluorescence signal formed by horseradish peroxidase catalysis for tumor-related exosome determination.Mikrochimica acta · 2020Article
- Exosome-Based Liquid Biopsy in Early Screening and Diagnosis of Cancers.Dose-response : a publication of International Hormesis SocietyReview
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Exosomes represent a new generation of biomarkers for the early diagnosis of hepatic carcinoma. A fluorometric assay is presented that is based on the hybridization chain reaction and magnetic nanoparticles for the highly sensitive determination of exosome (from HepG2 cells). Antibody as the recognition element was modified on the surface of magnetic nanoparticles. Antibody was used to capture the exosome. The Probe 1 was consisted of aptamer sequence and trigger sequence. Aptamer sequence will bind with the surface protein of exosome. The trigger sequence will hybridize with the probe2 (FAM-labeled) and the probe3 (FAM-labeled). So the product of the hybridization chain reaction will present a strong fluorescence signal. The fluorescence product of hybridization chain reaction will link with magnetic nanoparticles through the 'magnetic nanoparticles-antibody-exosome-aptamer' structure. The product can be separated from the matrix due to the present of the magnetic nanoparticles. The excitation was set at 490 nm. The fluorescence value of the emission spectra at 519 nm was set as the signal response. The linear range of this assay is from 1000 to 10
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