ArticleNucleic acids research2020
Structural insights into target DNA recognition by R2R3-MYB transcription factors.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 64 citations in OpenAlex.
- Molecular and transcriptional regulation of plant defense responses to aphid infestation.Stress biology · 2026Review
- Evolutionary and functional divergence of MYB regulatory networks in flavonoid biosynthesis across Poaceae, non-Poaceae monocots, and eudicot horticultural plants.Horticulture research · 2026Review
- DNA hypomethylation enables the transcriptional repressor SlSPL-CNR to control fruit flavor ester biosynthesis.Nature communications · 2026Article
- Genome-wide identification of the MYB gene family and identification of candidate genes that involved in anthocyanin synthesis in cowpea (Vigna unguiculata [L.] Walp).BMC plant biology · 2026Article
- A single nucleotide mutation in FngMYB10 causes white fruit phenotype in Fragaria nilgerrensis.BMC plant biology · 2026Article
- Arabidopsis TRB Proteins Form Two Closely Related Complexes to Mediate H3K4me3 Demethylation and Transcriptional Repression.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A natural allele of the transcription factor gene TaMYB-D7b is a genetic signature for phosphorus deficiency in wheat.Plant physiology · 2025Article
- Phylogenetic and functional analysis of MYB genes unraveling its role involved in anthocyanin biosynthesis in H macrophylla.Scientific reports · 2025Article
- A 3R-MYB transcription factor is involved in Methyl Jasmonate-Induced disease resistance in Agaricus bisporus and has implications for disease resistance in Arabidopsis.Journal of advanced research · 2025Article
- Biosynthesis and Regulatory Mechanisms of Plant Flavonoids: A Review.Plants (Basel, Switzerland) · 2025Review
- Symmetric adenine methylation is an essential DNA modification in the early-diverging fungus Rhizopus microsporus.Nature communications · 2025Article
- GhLPF1 Associated Network Is Involved with Cotton Lint Percentage Regulation Revealed by the Integrative Analysis of Spatial Transcriptome.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
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- Epigenetic gene regulation in plants and its potential applications in crop improvement.Nature reviews. Molecular cell biology · 2025Review
- Genome Sequencing and Metabolic Potential Analysis ofJournal of fungi (Basel, Switzerland) · 2024Article
- MYB transcription factors in Peucedanum Praeruptorum Dunn: the diverse roles of the R2R3-MYB subfamily in mediating coumarin biosynthesis.BMC plant biology · 2024Article
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- T and Z, partial seed coat patterning genes in common bean, provide insight into the structure and protein interactions of a plant MBW complex.G3 (Bethesda, Md.) · 2024Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As the largest group of MYB family transcription factors, R2R3-MYB proteins play essential roles during plant growth and development. However, the structural basis underlying how R2R3-MYBs recognize the target DNA remains elusive. Here, we report the crystal structure of Arabidopsis WEREWOLF (WER), an R2R3-MYB protein, in complex with its target DNA. Structural analysis showed that the third α-helices in both the R2 and R3 repeats of WER fit in the major groove of the DNA, specifically recognizing the DNA motif 5'-AACNGC-3'. In combination with mutagenesis, in vitro binding and in vivo luciferase assays, we showed that K55, N106, K109 and N110 are critical for the function of WER. Although L59 of WER is not involved in DNA binding in the structure, ITC analysis suggested that L59 plays an important role in sensing DNA methylation at the fifth position of cytosine (5mC). Like 5mC, methylation at the sixth position of adenine (6mA) in the AAC element also inhibits the interaction between WER and its target DNA. Our study not only unravels the molecular basis of how WER recognizes its target DNA, but also suggests that 5mC and 6mA modifications may block the interaction between R2R3-MYB transcription factors and their target genes.
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