ArticleJournal of assisted reproduction and genetics2020
Role of the PI3K and Hippo pathways in follicle activation after grafting of human ovarian tissue.
Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 67 citations in OpenAlex.
- Enhancement of angiogenesis and follicular survival using growth factors during ovarian tissue transplantation: a systematic review.Archives of gynecology and obstetrics · 2025Pooled it
- DNMT/TET Imbalance and Network-Level DNA Methylation Remodeling in Ovarian Aging: Mechanistic Perspectives.Biology · 2026Review
- Unveiling the role of the PI3K/Akt signaling pathway in follicular development and non-malignant ovarian dysfunction: a comprehensive review.Journal of ovarian research · 2026Review
- Dynamics of YAP localization and transcript activity in human oocytes and granulosa cells across early-stage folliculogenesis: an exploratory investigation.Journal of assisted reproduction and genetics · 2025Article
- The mechanism and protective strategies of follicle injury after ovarian tissue cryopreservation and thawed transplantation: a review.Journal of ovarian research · 2025Review
- Construction and Bioengineering of Human Bioprosthetic Ovaries from Cryopreserved Ovarian Tissue.International journal of molecular sciences · 2025Review
- How to Preserve Fertility in Reproductive-Age Women with Cancer.Journal of clinical medicine · 2025Review
- Ovarian Mechanobiology: Understanding the Interplay Between Mechanics and Follicular Development.Cells · 2025Review
- Electroacupuncture enhances the mitophagy of granulosa cells in premature ovarian insufficiency model mice by inactivating the hippo-yes-associated protein/transcriptional co-activator with postsynaptic density protein, drosophila disc large tumor suppressor, and zonula occludens-1 protein binding motif pathway.Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan · 2025Article
- Advances in ovarian follicle culture systems: exploring the interplay between cells, matrix, and ovarian architecture.Animal reproduction · 2025Article
- Transcription repression of estrogen receptor alpha by ghrelin/Gq/11/YAP signaling in granulosa cells promotes polycystic ovary syndrome.Human cell · 2024Article
- Adolescent exposure to a mixture of per- and polyfluoroalkyl substances (PFAS) depletes the ovarian reserve, increases ovarian fibrosis, and alters the Hippo pathway in adult female mice.Toxicological sciences : an official journal of the Society of Toxicology · 2024Article
- Review
- Current Fertility Preservation Steps in Young Women Suffering from Cancer and Future Perspectives.International journal of molecular sciences · 2024Review
- Research progress on mechanism of follicle injury after ovarian tissue transplantation and protective strategies.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2024Review
- Effects of Regulating Hippo and Wnt on the Development and Fate Differentiation of Bovine Embryo.International journal of molecular sciences · 2024Article
- Comprehensive Review of In Vitro Human Follicle Development for Fertility Restoration: Recent Achievements, Current Challenges, and Future Optimization Strategies.Journal of clinical medicine · 2024Review
- Impact of human ovarian tissue manipulation on follicles: evidence of a potential first wave of follicle activation during fertility preservation procedures.Journal of assisted reproduction and genetics · 2023Article
- The mTOR Inhibitor Rapamycin Counteracts Follicle Activation Induced by Ovarian Cryopreservation in Murine Transplantation Models.Medicina (Kaunas, Lithuania) · 2023Article
- Rehmannioside D mitigates disease progression in rats with experimental-induced diminished ovarian reserveThe Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
purposeOur aim was to elucidate the mechanisms involved in follicle activation of the ovarian reserve after human ovarian tissue transplantation, with specific focus on the role of the effectors of the PI3K (mTOR and FOXO1) and Hippo (YAP) signaling pathways and whether they are somehow altered.
methodsFrozen-thawed ovarian tissue was collected from six women (age 25-35 years) undergoing surgery for non-ovarian pathologies and divided into 4 fragments in each case: one for non-grafted controls and three for grafting to immunodeficient mice for 3, 7 and 21 days. The tissue was processed for hematoxylin and eosin staining, immunohistochemistry and immunofluorescence at different timepoints before and after grafting. Activation of the PI3K and Hippo signaling pathways was investigated by analysis of mTOR phosphorylation, FOXO1 cytoplasmic localization and YAP nuclear localization.
resultsNo change in mTOR levels was observed in primordial follicles post-transplantation, but a significant upturn was recorded in growing follicles compared with primordial follicles, irrespective of grafting time. A higher percentage of primordial follicles was also found with FOXO1 in the cytoplasm after 3 days of transplantation than in non-grafted controls. Finally, a greater proportion of primordial follicles was detected with YAP in the nucleus at all timepoints after grafting.
conclusionsThis study supports the hypothesis that follicle activation may occur as an early event after transplantation, with follicle growth and death both contributing to the burnout phenomenon. This is the first time that the effectors of the PI3K and Hippo pathways have been investigated in grafted human ovarian tissue and their role in burnout documented.
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Registered trials
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