ArticleClinical infectious diseases : an official publication of the Infectious Diseases Society of America2020
Isolation of Mycobacterium lepromatosis and Development of Molecular Diagnostic Assays to Distinguish Mycobacterium leprae and M. lepromatosis.
Article in Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 3 syntheses or guidelines pooled it, 69 citations in OpenAlex.
- Silence on the plate: revisiting the enigma ofFrontiers in microbiology · 2025Pooled it
- An update on leprosy immunopathogenesis: systematic review.Frontiers in immunology · 2024Pooled it
- Systematic Review of Hansen Disease Attributed to Mycobacterium lepromatosis.Emerging infectious diseases · 2023Pooled it
- UndetectableOne health (Amsterdam, Netherlands) · 2026Article
- Identification of Mycobacterium leprae and Mycobacterium lepromatosis Cases with Broad-Range Molecular Assays at a Single Large Reference Laboratory.The American journal of tropical medicine and hygiene · 2026Article
- M. leprae, M. lepromatosis, and Leprosy: Surprises and Surmises.The American journal of tropical medicine and hygiene · 2026Article
- A Multiepitope Protein Based on Selected Synthetic Peptides Enables Effective Serological Screening of Exposure toACS omega · 2026Article
- British Red Squirrels (Interdisciplinary perspectives on infectious diseases · 2026Article
- 4,000-year-old Mycobacterium lepromatosis genomes from Chile reveal long establishment of Hansen's disease in the Americas.Nature ecology & evolution · 2025Article
- Vasculonecrotic Reaction Caused by Mycobacterium Lepromatosis Infection-A Case Report of an HIV/Leprosy-Coinfected Patient.Infectious disease reports · 2025Article
- Leprosy.Nature reviews. Disease primers · 2024Review
- Multiple Papules and Nodules Over the Body with Diffuse Thickening of the Facial Skin: A Quiz.Acta dermato-venereologica · 2024Article
- Molecular and Serological Surveillance forAnimals : an open access journal from MDPI · 2024Article
- A case report and literature review: Mycobacterium leprae infection diagnosed by metagenomic next-generation sequencing of cerebrospinal fluid.BMC infectious diseases · 2024Review
- Fever of unknown origin, blood and cerebrospinal fluid involvement: a leprosy case report.Frontiers in immunology · 2024Article
- High seropositivity against NDO-LID in a group of household contacts of leprosy patients. Are we close to leprosy elimination in Colombia?Pathogens and global health · 2023Observational
- Mycobacterium leprae in Armadillo Tissues from Museum Collections, United States.Emerging infectious diseases · 2023Article
- Leprosy in an Adopted Woman Diagnosed by Molecular Tools: A Case Report from a Non-Endemic Area.Pathogens (Basel, Switzerland) · 2023Article
- Psoriasis and Leprosy: An Arcane Relationship.Journal of inflammation research · 2023Article
- Next-generation sequencing-assisted diagnosis of a case of leprosy misdiagnosed as erythema multiforme.Annals of clinical microbiology and antimicrobials · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors at 6 institutions in 3 countries.
Funding
Abstract
backgroundMycobacterium leprae was thought to be the exclusive causative agent of leprosy until Mycobacterium lepromatosis was identified in a rare form of leprosy known as diffuse lepromatous leprosy (DLL).
methodsWe isolated M. lepromatosis from a patient with DLL and propagated it in athymic nude mouse footpads. Genomic analysis of this strain (NHDP-385) identified a unique repetitive element, RLPM, on which a specific real-time quantitative polymerase chain reaction assay was developed. The RLPM assay, and a previously developed RLEP quantitative polymerase chain reaction assay for M. leprae, were validated as clinical diagnostic assays according to Clinical Laboratory Improvement Amendments guidelines. We tested DNA from archived histological sections, patient specimens from the United States, Philippines, and Mexico, and US wild armadillos.
resultsThe limit of detection for the RLEP and RLPM assays is 30 M. leprae per specimen (0.76 bacilli per reaction; coefficient of variation, 0.65%-2.44%) and 122 M. lepromatosis per specimen (3.05 bacilli per reaction; 0.84%-2.9%), respectively. In histological sections (n = 10), 1 lepromatous leprosy (LL), 1 DLL, and 3 Lucio reactions contained M. lepromatosis; 2 LL and 2 Lucio reactions contained M. leprae; and 1 LL reaction contained both species. M. lepromatosis was detected in 3 of 218 US biopsy specimens (1.38%). All Philippines specimens (n = 180) were M. lepromatosis negative and M. leprae positive. Conversely, 15 of 47 Mexican specimens (31.91%) were positive for M. lepromatosis, 19 of 47 (40.43%) were positive for M. leprae, and 2 of 47 (4.26%) contained both organisms. All armadillos were M. lepromatosis negative.
conclusionsThe RLPM and RLEP assays will aid healthcare providers in the clinical diagnosis and surveillance of leprosy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.