ArticleNature protocols2019
Imaging neuromodulators with high spatiotemporal resolution using genetically encoded indicators.
Article in Nature protocols, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 66 citations in OpenAlex.
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- Histamine Dynamics During Ingestive Behavior Measured by the Novel Biosensor HisLightG.Journal of neurochemistry · 2025Article
- Genetically encoded sensors illuminateIBRO neuroscience reports · 2025Article
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- Intersection of GPCR trafficking and cAMP signaling at endomembranes.The Journal of cell biology · 2025Review
- Rapid fluctuations in histamine associated with intake of nutritive and non-nutritive solutions.bioRxiv : the preprint server for biology · 2025Article
- Examining the role of the photopigment melanopsin in the striatal dopamine response to light.Frontiers in systems neuroscience · 2025Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
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- A chemogenetic approach for dopamine imaging with tunable sensitivity.Nature communications · 2024Article
- Changes in dorsomedial striatum activity during expression of goal-directed vs. habit-like cue-induced cocaine seeking.Addiction neuroscience · 2024Article
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- The physiological control of eating: signals, neurons, and networks.Physiological reviews · 2022 · on this mapArticle
- Ratiometric Imaging of Mitochondrial Hydrogen Peroxide in AβACS sensors · 2022Article
- Real-Time Image Processing Toolbox for All-Optical Closed-Loop Control of Neuronal Activities.Frontiers in cellular neuroscience · 2022Article
Corrections and comments
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Authors and funding
11 authors at 7 institutions in 2 countries.
Funding
Abstract
Multiple aspects of neural activity, from neuronal firing to neuromodulator release and signaling, underlie brain function and ultimately shape animal behavior. The recently developed and constantly growing toolbox of genetically encoded sensors for neural activity, including calcium, voltage, neurotransmitter and neuromodulator sensors, allows precise measurement of these signaling events with high spatial and temporal resolution. Here, we describe the engineering, characterization and application of our recently developed dLight1, a suite of genetically encoded dopamine (DA) sensors based on human inert DA receptors. dLight1 offers high molecular specificity, requisite affinity and kinetics and great sensitivity for measuring DA release in vivo. The detailed workflow described in this protocol can be used to systematically characterize and validate dLight1 in increasingly intact biological systems, from cultured cells to acute brain slices to behaving mice. For tool developers, we focus on characterizing five distinct properties of dLight1: dynamic range, affinity, molecular specificity, kinetics and interaction with endogenous signaling; for end users, we provide comprehensive step-by-step instructions for how to leverage fiber photometry and two-photon imaging to measure dLight1 transients in vivo. The instructions provided in this protocol are designed to help laboratory personnel with a broad range of experience (at the graduate or post-graduate level) to develop and utilize novel neuromodulator sensors in vivo, by using dLight1 as a benchmark.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.