ArticleMethods and protocols2019
A Method to Reuse Archived H&E Stained Histology Slides for a Multiplex Protein Biomarker Analysis.
Article in Methods and protocols, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Comparative pathophysiological modeling: the advantage of "two-hit" over "one-hit" in acute liver failure studies.In vitro cellular & developmental biology. Animal · 2026Article
- Destaining hematoxylin and eosin stains and restaining for immunohistochemistry has diagnostic value for cytology samples.CytoJournal · 2026Article
- Advanced tissue technologies of blood-brain barrier organoids as high throughput toxicity readouts in drug development.Heliyon · 2025Article
- Immune microenvironment-dependent effects of age-associatedFrontiers in cellular and infection microbiology · 2025Article
- Genome-wide RNA-Seq identifies TP53-mediated embryonic stem cells inhibiting tumor invasion and metastasis.Stem cell research & therapy · 2024Article
- An end-to-end workflow for nondestructive 3D pathology.Nature protocols · 2024Review
- An end-to-end workflow for non-destructive 3D pathology.bioRxiv : the preprint server for biology · 2023Article
- Restaining-based annotation for cancer histology segmentation to overcome annotation-related limitations among pathologists.Patterns (New York, N.Y.) · 2023Article
- Improving mitotic cell counting accuracy and efficiency using phosphohistone-H3 (PHH3) antibody counterstained with haematoxylin and eosin as part of breast cancer grading.Histopathology · 2023Article
- Flow chamber staining modality for real-time inspection of dynamic phenotypes in multiple histological stains.PloS one · 2023Article
- Repeat Brn3a immunolabeling rescues faded staining and improves detection of retinal ganglion cells.Experimental eye research · 2023Article
- Artificial intelligence for multimodal data integration in oncology.Cancer cell · 2022Review
- Heparin Protects Severe Acute Pancreatitis by Inhibiting HMGB-1 Active Secretion from Macrophages.Polymers · 2022Article
- Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm.International journal of molecular sciences · 2022Review
- Accuracy of whole slide image based image analysis is adversely affected by preanalytical factors such as stained tissue slide and paraffin block age.Journal of pathology informatics · 2022Article
- Pitfalls and Caveats in Applying Chromogenic Immunostaining to Histopathological Diagnosis.Cells · 2021Review
- Emodin-Conjugated PEGylation of FeInternational journal of nanomedicine · 2021Article
- Deep Learning of Histopathology Images at the Single Cell Level.Frontiers in artificial intelligence · 2021Review
- A Parallel Comparison Method of Early Gastric Cancer: The Light Transmission-Assisted Pathological Examination of Specimens of Endoscopic Submucosal Dissection.Frontiers in oncology · 2021Article
- Optimizing Re-staining Techniques for the Restoration of Faded Hematoxylin and Eosin-stained Histopathology Slides: A Comparative Study.The journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyArticle
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8 authors.
Funding
Abstract
Archived Hematoxylin and Eosin (H&E) stained pathology slides are routinely stored to index formalin-fixed paraffin-embedded (FFPE) sample tissue blocks. FFPE blocks are clinically annotated human tumor specimens that can be valuable in studies decades after the tissue is collected. If stored properly, they have the potential to yield a valuable number of serial sectioned slides for diagnostic or research purposes. However, some retrospective studies are limited in scope because the tissue samples have been depleted or not enough material is available in stored blocks for serial sections. The goal of these studies was to determine if archived H&E-stained slides can be directly reutilized by optimizing methods to de-stain and then re-stain the H&E stained slides to allow the detection of several biomarkers of interest using a conjugated antibody with chromogen multiplex immunohistochemistry procedure. This simple but innovative procedure, combined with image analysis techniques, demonstrates the ability to perform precise detection of relevant markers correlated to disease progression in initially identified tumor regions in tissue. This may add clinical value in retaining H&E slides for further use.
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