ArticlePlant biotechnology journal2020
Genome editing in wheat microspores and haploid embryos mediated by delivery of ZFN proteins and cell-penetrating peptide complexes.
Article in Plant biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- Transgene-free genome editing in plants.aBIOTECH · 2026Review
- Advances in Genome Editing Through Haploid Induction Systems.International journal of molecular sciences · 2025Review
- Agrobacterium tumefaciens-Mediated Plant Transformation: A Review.Molecular biotechnology · 2024Review
- Targeted mutagenesis with sequence-specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects.The plant genome · 2023Review
- A bifunctional selectable marker for wheat transformation contributes to the characterization of male-sterile phenotype induced by a synthetic Ms2 gene.Plant cell reports · 2023Article
- Cell-Penetrating Peptides for Use in Development of Transgenic Plants.Molecules (Basel, Switzerland) · 2023Review
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- Applications of In Vitro Tissue Culture Technologies in Breeding and Genetic Improvement of Wheat.Plants (Basel, Switzerland) · 2022Review
- Article
- Applications of CPPs in Genome Editing of Plants.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Plant biomacromolecule delivery methods in the 21st century.Frontiers in genome editing · 2022Review
- Transgene-free genome editing and RNAi ectopic application in fruit trees: Potential and limitations.Frontiers in plant science · 2022Review
- Review
- Genome editing in cereal crops: an overview.Transgenic research · 2021Review
- Applicability of the EFSA Opinion on site-directed nucleases type 3 for the safety assessment of plants developed using site-directed nucleases type 1 and 2 and oligonucleotide-directed mutagenesis.EFSA journal. European Food Safety Authority · 2020Article
- Genome editing in wheat microspores and haploid embryos mediated by delivery of ZFN proteins and cell-penetrating peptide complexes.Plant biotechnology journal · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent advances in genome engineering technologies based on designed endonucleases (DE) allow specific and predictable alterations in plant genomes to generate value-added traits in crops of choice. The EXZACT Precision technology, based on zinc finger nucleases (ZFN), has been successfully used in the past for introduction of precise mutations and transgenes to generate novel and desired phenotypes in several crop species. Current methods for delivering ZFNs into plant cells are based on traditional genetic transformation methods that result in stable integration of the nuclease in the genome. Here, we describe for the first time, an alternative ZFN delivery method where plant cells are transfected with ZFN protein that eliminates the need for stable nuclease genomic integration and allows generation of edited, but not transgenic cells or tissues. For this study, we designed ZFNs targeting the wheat IPK1 locus, purified active ZFN protein from bacterial cultures, complexed with cell-penetrating peptides (CPP) and directly transfected the complex into either wheat microspores or embryos. NGS analysis of ZFN-treated material showed targeted edits at the IPK1 locus in independent experiments. This is the first description of plant microspore genome editing by a ZFN when delivered as a protein complexed with CPP.
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