ArticleNature communications2019
Adaptive prospective optical gating enables day-long 3D time-lapse imaging of the beating embryonic zebrafish heart.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Technological innovations and high-throughput applications of light-sheet microscopy.Biophysics reports · 2026Article
- Emerging Light-Sheet Fluorescence Microscopy for Cardiac Vascular Imaging in Zebrafish.Current treatment options in cardiovascular medicine · 2026Review
- Volumetric imaging and computation to explore contractile function in zebrafish hearts.Cell reports methods · 2025Article
- Multifunctional robotic micromanipulation system for automated cardiovascular disease therapy using zebrafish.npj biomedical innovations · 2025Article
- Multiscale and recursive unmixing of spatiotemporal rhythms for live-cell and intravital cardiac microscopy.Nature cardiovascular research · 2025Article
- morphoHeart: A quantitative tool for integrated 3D morphometric analyses of heart and ECM during embryonic development.PLoS biology · 2025Article
- Early heart development: examining the dynamics of function-form emergence.Biochemical Society transactions · 2024Review
- 4D Light-sheet imaging and interactive analysis of cardiac contractility in zebrafish larvae.APL bioengineering · 2023Article
- Application of transgenic zebrafish for investigating inflammatory responses to nanomaterials: Recommendations for new users.F1000Research · 2023Review
- Cardiotoxicity of Zebrafish Induced by 6-Benzylaminopurine Exposure and Its Mechanism.International journal of molecular sciences · 2022Article
- Macrophages trigger cardiomyocyte proliferation by increasing epicardial vegfaa expression during larval zebrafish heart regeneration.Developmental cell · 2022Article
- Selective Cdk9 inhibition resolves neutrophilic inflammation and enhances cardiac regeneration in larval zebrafish.Development (Cambridge, England) · 2022Article
- Immune cells in cardiac repair and regeneration.Development (Cambridge, England) · 2022Review
- KHz-rate volumetric voltage imaging of the whole Zebrafish heart.Biophysical reports · 2022Article
- Modeling Human Cardiac Arrhythmias: Insights from Zebrafish.Journal of cardiovascular development and disease · 2022Review
- Deep learning-based framework for cardiac function assessment in embryonic zebrafish from heart beating videos.Computers in biology and medicine · 2021Article
- Review
- Novel Optics-Based Approaches for Cardiac Electrophysiology: A Review.Frontiers in physiology · 2021Review
- Highly swelling pH-responsive microgels for dual mode near infra-red fluorescence reporting and imaging.Nanoscale advances · 2020Article
- Validating the Paradigm That Biomechanical Forces Regulate Embryonic Cardiovascular Morphogenesis and Are Fundamental in the Etiology of Congenital Heart Disease.Journal of cardiovascular development and disease · 2020Review
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
9 authors.
Funding
Abstract
Three-dimensional fluorescence time-lapse imaging of the beating heart is extremely challenging, due to the heart's constant motion and a need to avoid pharmacological or phototoxic damage. Although real-time triggered imaging can computationally "freeze" the heart for 3D imaging, no previous algorithm has been able to maintain phase-lock across developmental timescales. We report a new algorithm capable of maintaining day-long phase-lock, permitting routine acquisition of synchronised 3D + time video time-lapse datasets of the beating zebrafish heart. This approach has enabled us for the first time to directly observe detailed developmental and cellular processes in the beating heart, revealing the dynamics of the immune response to injury and witnessing intriguing proliferative events that challenge the established literature on cardiac trabeculation. Our approach opens up exciting new opportunities for direct time-lapse imaging studies over a 24-hour time course, to understand the cellular mechanisms underlying cardiac development, repair and regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.