ArticleNature communications2019
Cohesin cleavage by separase is enhanced by a substrate motif distinct from the cleavage site.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Roles of meiotic and mitotic cohesin in stem cell gene regulation and genome stability.Molecular therapy. Nucleic acids · 2026Review
- Substrate recognition by human separase.Science advances · 2025Article
- Cohesin-mediated stabilization of the CCAN complex at kinetochores in mitosis.Current biology : CB · 2025Article
- SGO2 does not play an essential role in separase inhibition during meiosis I in mouse oocytes.PLoS biology · 2025Article
- Cohesin and condensin regulate chromosome topology and play an essential role in maintaining pluripotency in embryonic stem cells.Scientific reports · 2025Article
- Review
- Securin regulates the spatiotemporal dynamics of separase.The Journal of cell biology · 2025Article
- Nucleic Acids and Electrical Signals.Reviews of physiology, biochemistry and pharmacology · 2025Review
- Role of chromosomal cohesion and separation in aneuploidy and tumorigenesis.Cellular and molecular life sciences : CMLS · 2024Review
- Securin Regulates the Spatiotemporal Dynamics of Separase.bioRxiv : the preprint server for biology · 2023Article
- The molecular mechanisms of human separase regulation.Biochemical Society transactions · 2023Review
- Separase and Roads to Disengage Sister Chromatids during Anaphase.International journal of molecular sciences · 2023Review
- Mapping separase-mediated cleavage in situ.NAR genomics and bioinformatics · 2022Article
- Review
- Cdc48 influence on separase levels is independent of mitosis and suggests translational sensitivity of separase.Cell reports · 2022Article
- Article
- Article
- A prometaphase mechanism of securin destruction is essential for meiotic progression in mouse oocytes.Nature communications · 2021Article
- Structure and Function of the Separase-Securin Complex.Sub-cellular biochemistry · 2021Review
- Deciphering the modes of human separase inhibition by securin and CDK1-CCNB1.Molecular & cellular oncology · 2021Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Chromosome segregation begins when the cysteine protease, separase, cleaves the Scc1 subunit of cohesin at the metaphase-to-anaphase transition. Separase is inhibited prior to metaphase by the tightly bound securin protein, which contains a pseudosubstrate motif that blocks the separase active site. To investigate separase substrate specificity and regulation, here we develop a system for producing recombinant, securin-free human separase. Using this enzyme, we identify an LPE motif on the Scc1 substrate that is distinct from the cleavage site and is required for rapid and specific substrate cleavage. Securin also contains a conserved LPE motif, and we provide evidence that this sequence blocks separase engagement of the Scc1 LPE motif. Our results suggest that rapid cohesin cleavage by separase requires a substrate docking interaction outside the active site. This interaction is blocked by securin, providing a second mechanism by which securin inhibits cohesin cleavage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.