Evidence map›Paper›PMID 31728989›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Visualization and Quantification of Phagocytosis by Neutrophils.

Gaelen Guzman, Fikadu G Tafesse

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.1field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Neutrophils in cancer.Nature reviews. Cancer · 2026
    Review
  2. Sex-dependent dysregulation of human neutrophil responses by bisphenol A.Environmental health : a global access science source · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Gaelen GuzmanDepartment of Molecular Microbiology & Immunology, Oregon Health & Science University, Portland, OR, USA.
Fikadu G TafesseDepartment of Molecular Microbiology & Immunology, Oregon Health & Science University, Portland, OR, USA. tafesse@ohsu.edu.
Oregon Health & Science University · US

Funding

Determining the role of sphingolipids in Mycobacterium tuberculosis infectionR01AI141549 · NIAID · OREGON HEALTH & SCIENCE UNIVERSITY · PI TAFESSE, FIKADU G. · 2020 to 2025
$2.4M
Defining the role of host lipid repertoire in Mycobacterium tuberculosis infectionR21AI124225 · NIAID · OREGON HEALTH & SCIENCE UNIVERSITY · PI TAFESSE, FIKADU G. · 2017 to 2018
$424k
NIAID NIH HHS R01 AI141549NIAID NIH HHS R21 AI124225
6 · The paper itself

Abstract

Phagocytosis by phagocytes such as neutrophils is a crucial part of the host innate immune response against invading pathogens. Phagocytosis is a complex process that initiates with the binding of the particles on the cell surface of the phagocytes through the interaction of pattern recognition receptors with ligands on the surface of the pathogens. During this process, phagocytes undergo extensive membrane reorganization and cytoskeleton rearrangement at their cell surface. To gain better insight about the molecular mechanisms of this dynamic cellular process, visualization and quantification in a high-throughput manner is essential. Here, we describe a microscope-based method to visualize and quantify phagocytic uptake of pathogens (such as bacteria and fungi) and model particulates that are larger than 0.5 μm (such as Zymosan A and IgG-coated beads).

Indexed as

PhagocytosisBiomarkersCell DifferentiationCell LineCell TrackingFluorescent Antibody TechniqueHigh-Throughput Screening AssaysHumansImage Processing, Computer-AssistedNeutrophilsOptical ImagingPhagocytesBiomarkersHigh-content imagingHigh-throughputNeutrophilsPhagocytesPhagocytosisPhenotypic analysis

Identifiers

PMID31728989
PMCPMC10628851
OpenAlexW2986519063

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.