ArticleWorld journal of microbiology & biotechnology2019
Evolutionary engineering and molecular characterization of a caffeine-resistant Saccharomyces cerevisiae strain.
Article in World journal of microbiology & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- An mTOR inhibitor discovery system using drug-sensitized yeast.GeroScience · 2025Article
- Evolutionary engineering and molecular characterization of an antimycin A-resistant Saccharomyces cerevisiae strain: the key role of pleiotropic drug resistance (PDR1).FEMS yeast research · 2025Article
- Sterol-Targeted Laboratory Evolution Allows the Isolation of Thermotolerant and Respiratory-Competent Clones of the Industrial Yeast Saccharomyces cerevisiae.Microbial biotechnology · 2025Article
- Use of gain-of-function suppressor screening to elucidateFrontiers in microbiology · 2025Article
- Experimental evolution of Saccharomyces cerevisiae for caffeine tolerance alters multidrug resistance and target of rapamycin signaling pathways.G3 (Bethesda, Md.) · 2024Article
- Response mechanisms of different Saccharomyces cerevisiae strains to succinic acid.BMC microbiology · 2024Article
- Experimental evolution ofbioRxiv : the preprint server for biology · 2024Article
- General mechanisms of weak acid-tolerance and current strategies for the development of tolerant yeasts.World journal of microbiology & biotechnology · 2023Review
- FromJournal of fungi (Basel, Switzerland) · 2023Review
- Caffeine-tolerant mutations selected through an at-home yeast experimental evolution teaching lab.bioRxiv : the preprint server for biology · 2023Article
- Tyrosol induces multiple drug resistance in yeastFrontiers in microbiology · 2023Article
- Genomic, transcriptomic, and metabolic characterization of 2-Phenylethanol-resistantFrontiers in microbiology · 2023Article
- Caffeine-tolerant mutations selected through an at-home yeast experimental evolution teaching lab.microPublication biology · 2023Article
- Improvement of macrolactins production by the genetic adaptation of Bacillus siamensis A72 to saline stress via adaptive laboratory evolution.Microbial cell factories · 2022Article
- Physiological and Molecular Characterization of an Oxidative Stress-ResistantFrontiers in microbiology · 2022Article
- Nickel induced cell impairments are negatively regulated by the Tor1 kinase in Schizosaccharomyces pombe.World journal of microbiology & biotechnology · 2021Article
- Review
- Evolutionary Engineering of an Iron-ResistantMicroorganisms · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Caffeine is a naturally occurring alkaloid, where its major consumption occurs with beverages such as coffee, soft drinks and tea. Despite a variety of reports on the effects of caffeine on diverse organisms including yeast, the complex molecular basis of caffeine resistance and response has yet to be understood. In this study, a caffeine-hyperresistant and genetically stable Saccharomyces cerevisiae mutant was obtained for the first time by evolutionary engineering, using batch selection in the presence of gradually increased caffeine stress levels and without any mutagenesis of the initial population prior to selection. The selected mutant could resist up to 50 mM caffeine, a level, to our knowledge, that has not been reported for S. cerevisiae so far. The mutant was also resistant to the cell wall-damaging agent lyticase, and it showed cross-resistance against various compounds such as rapamycin, antimycin, coniferyl aldehyde and cycloheximide. Comparative transcriptomic analysis results revealed that the genes involved in the energy conservation and production pathways, and pleiotropic drug resistance were overexpressed. Whole genome re-sequencing identified single nucleotide polymorphisms in only three genes of the caffeine-hyperresistant mutant; PDR1, PDR5 and RIM8, which may play a potential role in caffeine-hyperresistance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.