ArticleAMB Express2019
Development of a multiplex PCR assay for the simultaneous and rapid detection of six pathogenic bacteria in poultry.
Article in AMB Express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Establishment of a RapidBiosensors · 2026Article
- Etiological and Pathological Investigation of Bacterial Pneumonia in Forest Musk Deer (Veterinary medicine international · 2026Article
- Frequency of Pseudomonas's Antibiotic Resistance and Virulence Genes in Poultry Meat.Veterinary medicine and science · 2025Article
- RPA-CRISPR/Cas12a-based detection ofFrontiers in veterinary science · 2025Article
- Assessment of the presence of multidrug-resistantHeliyon · 2024Article
- Exploring of spectrum beta lactamase producing multidrug-resistantVeterinary and animal science · 2024Article
- Ultra-sensitive fluorescent biosensor for multiple bacteria detection based on CDs/QDs@ZIF-8 and microfluidic fluidized bed.Mikrochimica acta · 2024Article
- Occurrence and molecular characterization of Escherichia coli strains isolated from black grouse (Lyrurus tetrix) from the Karkonosze National Park in Poland.BMC veterinary research · 2024Article
- Salmonella detection with LAMP and qPCR and identification of serovars of interest by multiplex qPCR in poultry carcasses.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Simultaneous and rapid detection of avian respiratory diseases of small poultry using multiplex reverse transcription-Polymerase Chain Reaction assay.Poultry science · 2023Article
- A Multiplex PCR Method for Simultaneous Detection of Infectious Laryngotracheitis Virus andVeterinary sciences · 2023Article
- Antimicrobial Resistance Profile of Common Foodborne Pathogens Recovered from Livestock and Poultry in Bangladesh.Antibiotics (Basel, Switzerland) · 2022Article
- Establishment and Application of a Multiplex PCR Assay for the Rapid Detection ofPathogens (Basel, Switzerland) · 2022Article
- Development of Multiplex PCR Assay for Screening of T6SS-5 Gene Cluster: TheDiagnostics (Basel, Switzerland) · 2022Article
- Genetic diversity and multidrug resistance of phylogenic groups B2 and D in InPEC and ExPEC isolated from chickens in Central China.BMC microbiology · 2022Article
- Article
- Evaluation of a multiplex PCR method for the detection of porcine parvovirus types 1 through 7 using various field samples.PloS one · 2021Article
- Effect of Antibiotics on the Colonization of Live AttenuatedFrontiers in veterinary science · 2021Article
- Establishment and Application of a Dual TaqMan Real-Time PCR Method forPolish journal of microbiology · 2020Article
- Virulence and resistance determinants inJournal of advanced veterinary and animal research · 2020Article
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Authors and funding
12 authors.
Funding
Abstract
Escherichia coli, Pasteurella multocida, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella spp. and Staphylococcus aureus are six bacterial pathogens of avian. However, these pathogens may cause many similar pathological changes, resulting in clinical isolates that are difficult to quickly and simultaneously detect and identify. Here, a multiplex polymerase chain reaction (m-PCR) assay is reported to rapidly identify targets genes (phoA, KMT1, ureR, toxA, invA, and nuc) of these six pathogens in clinical samples. Six pairs of specific primers were designed. The optimal reaction conditions, specificity, and sensitivity of the m-PCR assay were investigated. The results showed that betaine remarkably improved amplification of the target genes. Specific test results showed that all six pathogens were detected by the proposed m-PCR protocol without cross-amplification with viruses or parasites. Sensitivity test results showed that the m-PCR system could amplify the six target genes from bacterial genomes or cultures with template amounts of 500 pg or 2.8-8.6 × 10
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