ArticleJournal of experimental botany2020
Citrus PH4-Noemi regulatory complex is involved in proanthocyanidin biosynthesis via a positive feedback loop.
Article in Journal of experimental botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- The RcDREB1E-RcMYB28 module improves drought tolerance in Rosa chinensis via RcLAR/RcCORA1-mediated proanthocyanidin biosynthesis and stress responses.The Plant journal : for cell and molecular biology · 2026Article
- CitPH4 Confers Resistance to Citrus Canker by Activating Papain-Like Cysteine Protease.Plant biotechnology journal · 2026Article
- Molecular orchestration of malate and citrate metabolism: regulatory networks governing organic acid dynamics and fruit quality attributes.Horticulture research · 2026Article
- AP2 transcription factor CsAIL6 negatively regulates citric acid accumulation in citrus fruits by interacting with a WD40 protein CsAN11.Horticulture research · 2025Article
- CaMYBA-CaMYC-CaTTG1 complex activates the transcription of anthocyanin synthesis structural genes and regulates anthocyanin accumulation in pepper (Frontiers in plant science · 2025Article
- Distinctive acidity in citrus fruit is linked to loss of proanthocyanidin biosynthesis.iScience · 2024Article
- Cytochrome P450 CitCYP97B modulates carotenoid accumulation diversity by hydroxylating β-cryptoxanthin in Citrus.Plant communications · 2024Article
- Transcription factor CsTT8 promotes fruit coloration by positively regulating the methylerythritol 4-phosphate pathway and carotenoid biosynthesis pathway in citrus (Horticulture research · 2023Article
- Article
- Vaccinium as a comparative system for understanding of complex flavonoid accumulation profiles and regulation in fruit.Plant physiology · 2023Article
- Article
- CitrusHorticulture research · 2023Article
- Characterization of Highbush Blueberry (Current issues in molecular biology · 2023Article
- Flavonoids - flowers, fruit, forage and the future.Journal of the Royal Society of New Zealand · 2023Review
- Evolution and functional diversification of R2R3-MYB transcription factors in plants.Horticulture research · 2022Article
- The high-quality genome of pummelo provides insights into the tissue-specific regulation of citric acid and anthocyanin during domestication.Horticulture research · 2022Article
- Identification, Characterization and Expression Analysis of Anthocyanin Biosynthesis-relatedInternational journal of molecular sciences · 2021Article
- Advances in Biosynthesis and Biological Functions of Proanthocyanidins in Horticultural Plants.Foods (Basel, Switzerland) · 2020Review
- Citrus Taste Modification Potentials by Genetic Engineering.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proanthocyanidins (PAs; or condensed tannins) are a major class of flavonoids that contribute to citrus fruit quality. However, the molecular mechanism responsible for PA biosynthesis and accumulation in citrus remains unclear. Here, we identify a PH4-Noemi regulatory complex that regulates proanthocyanidin biosynthesis in citrus. Overexpression of PH4 or Noemi in citrus calli activated the expression of PA biosynthetic genes and significantly increased the PA content. Interestingly, Noemi was also shown to be up-regulated in CsPH4-overexpressing lines compared with wild-type calli. Simultaneously, CsPH4 partially complemented the PA-deficient phenotype of the Arabidopsis tt2 mutant and promoted PA accumulation in the wild-type. Further analysis revealed that CsPH4 interacted with Noemi, and together these proteins synergistically activated the expression of PA biosynthetic genes by directly binding to the MYB-recognizing elements (MRE) of the promoters of these genes. Moreover, CsPH4 could directly bind to the promoter of Noemi and up-regulate the expression of this gene. These findings explain how the CsPH4-Noemi regulatory complex contributes to the activation of PA biosynthetic genes via a positive feedback loop and provide new insights into the molecular mechanisms underlying PA biosynthesis, which can be effectively employed for metabolic engineering to improve citrus fruit quality.
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