ArticleNucleic acids research2020
FIN-Seq: transcriptional profiling of specific cell types from frozen archived tissue of the human central nervous system.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 16 citations in OpenAlex.
- Transcriptomic Challenges We Faced with Animal Models for Neurological Disorders.Current issues in molecular biology · 2026Review
- Cardiomyocyte-Specific bcl11b Knockout Causes Left Ventricular Noncompaction by Dysregulating pou3f2 and Titin.International journal of molecular sciences · 2026Article
- Identification of novel genes with enriched expression in human intermediate progenitors reveals a key role for CDKN3 in cortical development.Stem cell reports · 2026Article
- Single-cell and spatial transcriptomics: deciphering brain complexity in health and disease.Nature reviews. Neurology · 2023Review
- Meso-seq for in-depth transcriptomics in ultra-low amounts of FACS-purified neuronal nuclei.Cell reports methods · 2022Article
- Article
- Single nucleus transcriptome and chromatin accessibility of postmortem human pituitaries reveal diverse stem cell regulatory mechanisms.Cell reports · 2022Article
- Joint single-cell measurements of nuclear proteins and RNA in vivo.Nature methods · 2021Article
- Multi-species single-cell transcriptomic analysis of ocular compartment regulons.Nature communications · 2021Article
- Molecular Profiling Reveals Involvement of ESCO2 in Intermediate Progenitor Cell Maintenance in the Developing Mouse Cortex.Stem cell reports · 2021Article
- Glyoxal fixation facilitates transcriptome analysis after antigen staining and cell sorting by flow cytometry.PloS one · 2021Article
- Genomics at cellular resolution: insights into cognitive disorders and their evolution.Human molecular genetics · 2020Review
- Innate Immune Mechanisms to Protect Against Infection at the Human Decidual-Placental Interface.Frontiers in immunology · 2020Review
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Thousands of frozen, archived tissue samples from the human central nervous system (CNS) are currently available in brain banks. As recent developments in RNA sequencing technologies are beginning to elucidate the cellular diversity present within the human CNS, it is becoming clear that an understanding of this diversity would greatly benefit from deeper transcriptional analyses. Single cell and single nucleus RNA profiling provide one avenue to decipher this heterogeneity. An alternative, complementary approach is to profile isolated, pre-defined cell types and use methods that can be applied to many archived human tissue samples that have been stored long-term. Here, we developed FIN-Seq (Frozen Immunolabeled Nuclei Sequencing), a method that accomplishes these goals. FIN-Seq uses immunohistochemical isolation of nuclei of specific cell types from frozen human tissue, followed by bulk RNA-Sequencing. We applied this method to frozen postmortem samples of human cerebral cortex and retina and were able to identify transcripts, including low abundance transcripts, in specific cell types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.