ArticleScientific reports2019
A standalone bioreactor system to deliver compressive load under perfusion flow to hBMSC-seeded 3D chitosan-graphene templates.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 64 citations in OpenAlex.
- Biomaterials for intervertebral disc regeneration: Niche reprogramming, precision therapeutics, and structural reconstruction.Bioactive materials · 2027Review
- Insights into bone and cartilage responses to pulsed electromagnetic field stimulation: a review with quantitative comparisons.Frontiers in bioengineering and biotechnology · 2025Review
- Towards Stem Cell Therapy for Critical-Sized Segmental Bone Defects: Current Trends and Challenges on the Path to Clinical Translation.Journal of functional biomaterials · 2024Review
- Article
- Direct coupled electrical stimulation towards improved osteogenic differentiation of human mesenchymal stem/stromal cells: a comparative study of different protocols.Scientific reports · 2024Article
- Review
- Chitosan nanoparticle applications in dentistry: a sustainable biopolymer.Frontiers in chemistry · 2024Review
- Human Bone-Marrow-Derived Stem-Cell-Seeded 3D Chitosan-Gelatin-Genipin Scaffolds Show Enhanced Extracellular Matrix Mineralization When Cultured under a Perfusion Flow in Osteogenic Medium.Materials (Basel, Switzerland) · 2023Article
- Mesenchymal Stem Cell Culture within Perfusion Bioreactors Incorporating 3D-Printed Scaffolds Enables Improved Extracellular Vesicle Yield with Preserved Bioactivity.Advanced healthcare materials · 2023Article
- Open-source perfusion system for medium-scale fabrication of demineralized bone matrix chip grafts.HardwareX · 2023Article
- Advances in In Vitro and In Vivo Bioreactor-Based Bone Generation for Craniofacial Tissue Engineering.BME frontiers · 2023Review
- Translation of biophysical environment in bone into dynamic cell culture under flow for bone tissue engineering.Computational and structural biotechnology journal · 2023Review
- Mechanically Competent Chitosan-Based Bioadhesive for Tendon-to-Bone Repair.Advanced healthcare materials · 2022Article
- Fiber Thickness and Porosity Control in a Biopolymer Scaffold 3D Printed through a Converted Commercial FDM Device.Materials (Basel, Switzerland) · 2022Article
- Bioengineering Outlook on Cultivated Meat Production.Micromachines · 2022Review
- Bioengineered Living Bone Grafts-A Concise Review on Bioreactors and Production Techniques In Vitro.International journal of molecular sciences · 2022Review
- Review
- Design of a custom-made device for real-time optical measurement of differential mineral concentrations in three-dimensional scaffolds for bone tissue engineering.Royal Society open science · 2022Article
- Osteogenic and Angiogenic Synergy of Human Adipose Stem Cells and Human Umbilical Vein Endothelial Cells Cocultured in a Modified Perfusion Bioreactor.Organogenesis · 2021Article
- The Effects of a Short Self-Assembling Peptide on the Physical and Biological Properties of Biopolymer Hydrogels.Pharmaceutics · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The availability of engineered biological tissues holds great potential for both clinical applications and basic research in a life science laboratory. A prototype standalone perfusion/compression bioreactor system was proposed to address the osteogenic commitment of stem cells seeded onboard of 3D chitosan-graphene (CHT/G) templates. Testing involved the coordinated administration of a 1 mL/min medium flow rate together with dynamic compression (1% strain at 1 Hz; applied twice daily for 30 min) for one week. When compared to traditional static culture conditions, the application of perfusion and compression stimuli to human bone marrow stem cells using the 3D CHT/G template scaffold induced a sizable effect. After using the dynamic culture protocol, there was evidence of a larger number of viable cells within the inner core of the scaffold and of enhanced extracellular matrix mineralization. These observations show that our novel device would be suitable for addressing and investigating the osteogenic phenotype commitment of stem cells, for both potential clinical applications and basic research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.