Evidence map›Paper›PMID 31727936›Full record

ArticleScientific reports2019

Construction and characterization of a double mutant of Enterococcus faecalis that does not produce biogenic amines.

Marta Perez, Marina Calles-Enríquez, Beatriz Del Rio, Begoña Redruello, Anne de Jong, Oscar P Kuipers, Jan Kok, M Cruz Martin, Victor Ladero, Maria Fernandez and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 5 citations in OpenAlex.

  1. Combined Genome-Wide and Phenotypic Profiling ofFoods (Basel, Switzerland) · 2026
    Article
  2. Review
  3. Molecular Identification and Biogenic Amine Production Capacity ofInternational journal of molecular sciences · 2025
    Article
  4. Article
  5. Article
  6. GABA-ProducingFoods (Basel, Switzerland) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Marta PerezDairy Research Institute, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Rio Linares s/n, 33300, Villaviciosa, Spain.
Marina Calles-EnríquezDairy Research Institute, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Rio Linares s/n, 33300, Villaviciosa, Spain.
Beatriz Del RioDairy Research Institute, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Rio Linares s/n, 33300, Villaviciosa, Spain.ORCID http://orcid.org/0000-0001-8107-1975
Begoña RedruelloDairy Research Institute, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Rio Linares s/n, 33300, Villaviciosa, Spain.
Anne de JongDepartment of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747, AG, Groningen, The Netherlands.
Oscar P KuipersDepartment of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747, AG, Groningen, The Netherlands.
Jan KokDepartment of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747, AG, Groningen, The Netherlands.
M Cruz MartinDairy Research Institute, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Rio Linares s/n, 33300, Villaviciosa, Spain.
Victor LaderoDairy Research Institute, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Rio Linares s/n, 33300, Villaviciosa, Spain.ORCID http://orcid.org/0000-0002-7613-3745
Maria FernandezDairy Research Institute, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Rio Linares s/n, 33300, Villaviciosa, Spain. mfernandez@ipla.csic.es.
Miguel A AlvarezDairy Research Institute, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Rio Linares s/n, 33300, Villaviciosa, Spain.ORCID http://orcid.org/0000-0001-9607-7480
Consejo Superior de Investigaciones Científicas · ESUniversity of Groningen · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enterococcus faecalis is a lactic acid bacterium characterized by its tolerance of very diverse environmental conditions, a property that allows it to colonize many different habitats. This species can be found in food products, especially in fermented foods where it plays an important role as a biopreservative and influences the development of organoleptic characteristics. However, E. faecalis also produces the biogenic amines tyramine and putrescine. The consumption of food with high concentrations of these compounds can cause health problems. The present work reports the construction, via homologous recombination, of a double mutant of E. faecalis in which the clusters involved in tyramine and putrescine synthesis (which are located in different regions of the chromosome) are no longer present. Analyses showed the double mutant to grow and adhere to intestinal cells normally, and that the elimination of genes involved in the production of tyramine and putrescine has no effect on the expression of other genes.

Indexed as

Gene DeletionGene Expression Regulation, BacterialMultigene FamilyBacterial AdhesionBiofilmsCaco-2 CellsChromosomes, BacterialEnterococcus faecalisFood MicrobiologyGenetic EngineeringHomologous RecombinationHumansHydrogen-Ion ConcentrationPutrescineTranscriptomeTyraminePutrescineTyramine

Identifiers

PMID31727936
PMCPMC6856193
OpenAlexW2985520823

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.