Evidence map›Paper›PMID 31727926›Full record

ArticleScientific reports2019

An evaluation of the SureID 23comp Human Identification Kit for kinship testing.

Hussain M Alsafiah, Ali A Aljanabi, Sibte Hadi, Saleh S Alturayeif, William Goodwin

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.3field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 2 countries.

Hussain M AlsafiahSchool of Forensic and Applied Sciences, University of Central Lancashire, Preston, United Kingdom. hmhalsafiah@uclan.ac.uk.ORCID http://orcid.org/0000-0002-4580-5537
Ali A AljanabiSchool of Forensic and Applied Sciences, University of Central Lancashire, Preston, United Kingdom.
Sibte HadiSchool of Forensic and Applied Sciences, University of Central Lancashire, Preston, United Kingdom.ORCID http://orcid.org/0000-0002-2994-3083
Saleh S AlturayeifForensic Genetics Laboratory, General Administration of Criminal Evidences, Public Security, Ministry of Interior, Riyadh, Saudi Arabia.
William GoodwinSchool of Forensic and Applied Sciences, University of Central Lancashire, Preston, United Kingdom. whgoodwin@uclan.ac.uk.ORCID http://orcid.org/0000-0002-3632-3552
University of Lancashire · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Short tandem repeat (STR) profiling has been routinely used in kinship testing since the introduction of commercial kits in the mid-1990s. While 15 to 23 STR loci normally give definitive results in simple kinship testing, additional loci are sometimes required to resolve complex cases. The SureID 23comp Human Identification Kit, recently released by Health Gene Technologies (China), multiplexes amelogenin and 22 autosomal STRs, 17 of which are non-CODIS STRs. This enables the profiling of 38-40 loci when used in conjunction with widely used commercial kits. In this study, the kit was evaluated for kinship applications as a supplementary STR kit following the minimum criteria for validation recommended by the European Network of Forensic Science Institutes (ENFSI) and the Scientific Working Group on DNA Analysis Methods (SWGDAM) using 500 samples. Performance was comparable with other commercial kits demonstrating: repeatability and reproducibility; precision (maximum s.d. 0.1048 nt); accuracy, all alleles were within ±0.41 nt compared to the actual sizes; heterozygous peak balances at all loci >68%; stutter ratios ranged from 3.8% to 16.15%; full profiles were generated with 125 pg DNA (95.12% of alleles at 62 pg),; and we found 100% concordance over 5 common STRs with the GlobalFiler kit.

Indexed as

Genetic LociGenome, HumanMicrosatellite RepeatsAllelesAmelogeninDNAForensic AnthropologyForensic GeneticsGene FrequencyGenotyping TechniquesHeterozygoteHumansReproducibility of ResultsAmelogeninAMELY protein, humanDNA

Identifiers

PMID31727926
PMCPMC6856129
OpenAlexW2987922231

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.