ArticleScientific reports2019
Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Article
- Control theory analysis of dynamic metabolic response elucidates mitochondrial-cytoplasmic coupling and nutrient partitioning.bioRxiv : the preprint server for biology · 2026Article
- Mitochondrial adaptations from heat acclimation - A narrative review.Journal of thermal biology · 2025Review
- Oocyte Exposure to Low Levels of Triclosan Has a Significant Impact on Subsequent Embryo Physiology.International journal of environmental research and public health · 2025Article
- In Vitro Maturation of Bovine Oocytes in the Presence of Resveratrol and Ellagic Acid but Not Chlorogenic Acid Modulates Blastocyst Antioxidant Gene Expression Without Impacting Embryo Development and Oxygen Consumption.Antioxidants (Basel, Switzerland) · 2025Article
- The Different Responsiveness of C3- and C5-deficient Murine BM Cells to Oxidative Stress Explains Why C3 Deficiency, in Contrast to C5 Deficiency, Correlates with Better Pharmacological Mobilization and Engraftment of Hematopoietic Cells.Stem cell reviews and reports · 2025Article
- Gold complex QB1561 suppresses drug-resistant cancer cells by inhibiting TrxR and mitochondrial respiratory function.Frontiers in pharmacology · 2025Article
- Real-time assessment of relative mitochondrial ATP synthesis response against inhibiting and stimulating substrates (MitoRAISE).Cancer & metabolism · 2024Article
- Determinants of thermal homeostasis in the preimplantation embryo: a role for the embryo's central heating system?Journal of assisted reproduction and genetics · 2024Review
- Dual-inhibition of NAMPT and PAK4 induces anti-tumor effects in 3D-spheroids model of platinum-resistant ovarian cancer.Cancer gene therapy · 2024Article
- Lack of detectable sex differences in the mitochondrial function of Caenorhabditis elegans.BMC ecology and evolution · 2024Article
- Metabolic Profiling of SH-SY5Y and Neuro2A Cells in Relation to Fetal Calf Serum (FCS) Concentration in Culture Media.Metabolites · 2024Article
- Mitochondrial Dysfunction in Advanced Maternal Aged Cumulus Cells: A Possible Link to ATP Synthase Impairment?Biomolecules · 2024Article
- Disruption of testis-enriched cytochrome c oxidase subunit COX6B2 but not COX8C leads to subfertility.Experimental animals · 2024Article
- The follicle-stimulating hormone triggers rapid changes in mitochondrial structure and function in porcine cumulus cells.Scientific reports · 2024Article
- Article
- Effect of lipotoxicity on mitochondrial function and epigenetic programming during bovine in vitro embryo production.Scientific reports · 2023Article
- The mitochondrial respiration signature of the bovine blastocyst reflects both environmental conditions of development as well as embryo quality.Scientific reports · 2023Article
- Acyl-Carnitines Exert Positive Effects on Mitochondrial Activity under Oxidative Stress in Mouse Oocytes: A Potential Mechanism Underlying Carnitine Efficacy on PCOS.Biomedicines · 2023Article
- Mitochondrial Dysfunction in PCOS: Insights into Reproductive Organ Pathophysiology.International journal of molecular sciences · 2023Review
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondria provide the major source of ATP for mammalian oocyte maturation and early embryo development. Oxygen Consumption Rate (OCR) is an established measure of mitochondrial function. OCR by mammalian oocytes and embryos has generally been restricted to overall uptake and detailed understanding of the components of OCR dedicated to specific molecular events remains lacking. Here, extracellular flux analysis (EFA) was applied to small groups of bovine, equine, mouse and human oocytes and bovine early embryos to measure OCR and its components. Using EFA, we report the changes in mitochondrial activity during the processes of oocyte maturation, fertilisation, and pre-implantation development to blastocyst stage in response to physiological demands in mammalian embryos. Crucially, we describe the real time partitioning of overall OCR to spare capacity, proton leak, non-mitochondrial and coupled respiration - showing that while activity changes over the course of development in response to physiological demand, the overall efficiency is unchanged. EFA is shown to be able to measure mitochondrial function in small groups of mammalian oocytes and embryos in a manner which is robust, rapid and easy to use. EFA is non-invasive and allows real-time determination of the impact of compounds on OCR, facilitating an assessment of the components of mitochondrial activity. This provides proof-of-concept for EFA as an accessible system with which to study mammalian oocyte and embryo metabolism.
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