ArticleCell communication and signaling : CCS2019
Specific trophoblast transcripts transferred by extracellular vesicles affect gene expression in endometrial epithelial cells and may have a role in embryo-maternal crosstalk.
Article in Cell communication and signaling : CCS, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 61 citations in OpenAlex.
- Exploration of extracellular vesicles from porcine endometrial cells for enhancing the development of parthenogenetic embryos.Veterinary and animal science · 2026Article
- Endometrial cortisol level and its relationship with psychological stress, molecular tissue changes, and clinical outcomes in infertile women.Scientific reports · 2026Article
- Detection of Organelle-Specific Dyes Labeled Extracellular Vesicles with Colocalization-Fluorescence Nanoparticle Tracking Analysis.ACS omega · 2026Article
- Does extracellular vesicle specificity truly exist?Cell communication and signaling : CCS · 2026Review
- Extracellular Vesicles in Reproduction: Biology, Production, and Potential Applications in Livestock Breeding.Reproduction in domestic animals = Zuchthygiene · 2025Review
- Menstrual blood serum extracellular vesicles reveal novel molecular biomarkers and potential endotypes of unexplained infertility.Scientific reports · 2025Article
- Mir-218-5p from Extracellular Vesicles of Endometrium in Patients with Recurrent Implantation Failure Impairs Pre-Implantation Embryo Development.International journal of nanomedicine · 2025Article
- Rapid increase of MFGE8 secretion from endometrial epithelial cells is an indicator of extracellular vesicle mediated embryo maternal dialogue.Scientific reports · 2024Article
- From Germ Cells to Implantation: The Role of Extracellular Vesicles.Journal of developmental biology · 2024Review
- The evolving roles of extracellular vesicles in embryo-maternal communication.Communications biology · 2024Review
- Extracellular Vesicles in Pathophysiology: A Prudent Target That Requires Careful Consideration.Cells · 2024Review
- When the Embryo Meets the Endometrium: Identifying the Features Required for Successful Embryo Implantation.International journal of molecular sciences · 2024Review
- Do extracellular vesicles have specific target cells?; Extracellular vesicle mediated embryo maternal communication.Frontiers in molecular biosciences · 2024Article
- Effect of 3D and 2D cell culture systems on trophoblast extracellular vesicle physico-chemical characteristics and potency.Frontiers in cell and developmental biology · 2024Article
- Extracellular vesicles secreted by human aneuploid embryos present a distinct transcriptomic profile and upregulate MUC1 transcription in decidualised endometrial stromal cells.Human reproduction open · 2024Article
- Current knowledge on the role of extracellular vesicles in endometrial receptivity.European journal of medical research · 2023Review
- Spermatozoa, acts as an external cue and alters the cargo and production of the extracellular vesicles derived from oviductal epithelial cells in vitro.Journal of cell communication and signaling · 2023Article
- Secretory Proteomic Responses of Endometrial Epithelial Cells to Trophoblast-Derived Extracellular Vesicles.International journal of molecular sciences · 2023Article
- Characterization of bovine uterine fluid extracellular vesicles proteomic profiles at follicular and luteal phases of the oestrous cycle.Veterinary research communications · 2023Article
- Rhythm of the First Language: Dynamics of Extracellular Vesicle-Based Embryo-Maternal Communication in the Pre-Implantation Microenvironment.International journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSuccessful establishment of pregnancy hinges on appropriate communication between the embryo and the uterus prior to implantation, but the nature of this communication remains poorly understood. Here, we tested the hypothesis that the endometrium is receptive to embryo-derived signals in the form of RNA.
methodsWe have utilized a non-contact co culture system to simulate the conditions of pre implantation environment of the uterus. We bioorthogonally tagged embryonic RNA and tracked the transferred transcripts to endometrium. Transferred transcripts were separated from endometrial transcripts and sequenced. Changes in endometrial transcripts were quantified using quantitative PCR.
resultsWe show that three specific transcripts are transferred to endometrial cells. We subsequently demonstrate a role of extracellular vesicles (EVs) in this process, as EVs obtained from cultured trophoblast spheroids incubated with endometrial cells induced down-regulation of all the three identified transcripts in endometrial cells. Finally, we show that EVs/nanoparticles captured from conditioned culture media of viable embryos as opposed to degenerating embryos induce ZNF81 down-regulation in endometrial cells, hinting at the functional importance of this intercellular communication.
conclusionUltimately, our findings demonstrate the existence of an RNA-based communication which may be of critical importance for the establishment of pregnancy.
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