Evidence map›Paper›PMID 31727072›Full record

ArticleMicrobial cell factories2019

Restructured Lactococcus lactis strains with emergent properties constructed by a novel highly efficient screening system.

Fulu Liu, Yating Zhang, Wanjin Qiao, Duolong Zhu, Haijin Xu, Per Erik Joakim Saris, Mingqiang Qiao

Abstract read
In one paragraph

Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Advances in the biosynthesis of D-allulose.World journal of microbiology & biotechnology · 2024
    Review
  4. Construction and Analysis of Food-GradeJournal of microbiology and biotechnology · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Fulu LiuKey Laboratory of Molecular Microbiology and Technology, College of Life Sciences, Ministry of Education, Nankai University, Room 301, Tianjin, China.
Yating ZhangKey Laboratory of Molecular Microbiology and Technology, College of Life Sciences, Ministry of Education, Nankai University, Room 301, Tianjin, China.
Wanjin QiaoKey Laboratory of Molecular Microbiology and Technology, College of Life Sciences, Ministry of Education, Nankai University, Room 301, Tianjin, China.
Duolong ZhuDepartment of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
Haijin XuKey Laboratory of Molecular Microbiology and Technology, College of Life Sciences, Ministry of Education, Nankai University, Room 301, Tianjin, China.
Per Erik Joakim SarisDepartment of Food and Environmental Sciences, University of Helsinki, Helsinki, Finland.
Mingqiang QiaoKey Laboratory of Molecular Microbiology and Technology, College of Life Sciences, Ministry of Education, Nankai University, Room 301, Tianjin, China. qiaomq@nankai.edu.cn.ORCID http://orcid.org/0000-0002-7561-888X

Funding

National Natural Science Foundation of China (CN) 31770102Tianjin Key research Program of Application Foundation and Advanced Technology 17JCZDJC32200
6 · The paper itself

Abstract

backgroundAfter 2.83% genome reduction in Lactococcus lactis NZ9000, a good candidate host for proteins production was obtained in our previous work. However, the gene deletion process was time consuming and laborious. Here, we proposed a convenient gene deletion method suitable for large-scale genome reduction in L. lactis NZ9000.

resultsPlasmid pNZ5417 containing a visually selectable marker P

conclusionsThus, this study provides a convenient and efficient system for large-scale genome deletion in L. lactis through application of visually selectable marker, which could be helpful for rapid genome streamlining and generation of restructured L. lactis strains that can be used as cell factories.

Indexed as

BiotechnologyGene DeletionGenetic EngineeringGenetic MarkersGenome, BacterialLactococcus lactisPlasmidsProtein BiosynthesisGenetic MarkersLactococcus lactisLarge-scale genome deletionVisually selectable marker

Identifiers

PMID31727072
PMCPMC6854693

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.