Evidence map›Paper›PMID 31727036›Full record

ArticleBMC musculoskeletal disorders2019

The effect of a single consecutive volume aspiration on concentrated bone marrow from the proximal humerus for clinical application.

Lukas N Muench, Cameron Kia, Alexander Otto, Julian Mehl, Joshua B Baldino, Mark P Cote, Mary Beth McCarthy, Knut Beitzel, Augustus D Mazzocca

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Article in BMC musculoskeletal disorders, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Lukas N MuenchDepartment of Orthopaedic Sports Medicine, Technical University, Munich, Germany. lukas.muench@tum.de.
Cameron KiaDepartment of Orthopaedic Surgery, University of Connecticut, Farmington, CT, USA.
Alexander OttoDepartment of Orthopaedic Surgery, University of Connecticut, Farmington, CT, USA.
Julian MehlDepartment of Orthopaedic Sports Medicine, Technical University, Munich, Germany.
Joshua B BaldinoDepartment of Orthopaedic Surgery, University of Connecticut, Farmington, CT, USA.
Mark P CoteDepartment of Orthopaedic Surgery, University of Connecticut, Farmington, CT, USA.
Mary Beth McCarthyDepartment of Orthopaedic Surgery, University of Connecticut, Farmington, CT, USA.
Knut BeitzelDepartment of Orthopaedic Sports Medicine, Technical University, Munich, Germany.
Augustus D MazzoccaDepartment of Orthopaedic Surgery, University of Connecticut, Farmington, CT, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLow aspiration volumes have been recommended to allow for higher concentrations of progenitor cells during bone marrow harvesting. However, these guidelines then require multiple aspiration attempts in order to maximize cellular yield. The purpose of this study was to investigate the effect of a single, high-volume aspiration with four consecutive aliquots on the number of nucleated cells (NCs) and colony-forming units (CFUs) in concentrated bone marrow aspirate (cBMA) taken from the proximal humerus.

methodscBMA was taken from the proximal humerus of patients undergoing arthroscopic rotator cuff surgery. Four 12-mL double syringes were used consecutively from a single trocar to obtain four 10 cc aliquots. Each then underwent centrifugation to create a fractionated layer rich in nucleated cells. Following cellular separation, NCs were counted and CFUs were evaluated after incubation of 7-10 days. Cellular comparisons between each aliquot were performed along with their interaction with patient age and sex.

resultsTwenty-nine patients (55.9 ± 4.6 years) were included in this study. The number of NCs and CFUs showed significant differences between the four aliquots of aspirate, with the first 10 cc aliquot providing the highest amount (p < 0.001, respectively). No significant differences were found between the sum of the three sequential aliquots compared to the initial 10 cc sample. There were no significant differences between male and female patients (p > 0.05). Increasing age resulted in no significant decrease in the number of NCs and CFUs across the four consecutive aliquots (p > 0.05).

conclusionIn conclusion, while the initial aliquot provided the greatest number of nucleated cells and cultured CFUs, the addition of each sequential volume aspirate yielded similar amounts in total. This demonstrates the potential effectiveness of obtaining of higher volume aspirates from the proximal humerus during rotator cuff repair.

Indexed as

ArthroscopyCell ProliferationBone Marrow CellsCell CountCells, CulturedCell SeparationCentrifugationColony-Forming Units AssayFemaleHumansHumerusMaleMiddle AgedRotator CuffStem CellsSuctionBiologic augmentationBone marrow aspirationProximal humerusShoulder surgery

Identifiers

PMID31727036
PMCPMC6857344

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.