ArticleBMC plant biology2019
Functional characterization of a liverworts bHLH transcription factor involved in the regulation of bisbibenzyls and flavonoids biosynthesis.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 55 citations in OpenAlex.
- Identification and functional analysis of MeJA-responsive bHLH family genes in Taraxacum kok-saghyz.Journal of plant research · 2026Article
- Desiccation Tolerance in Moss and Liverwort: Insights into the Evolutionary Mechanisms of Terrestrialization.International journal of molecular sciences · 2026Review
- Integrative metabolomic and transcriptomic analyses reveal flavonoid biosynthesis pathway in Eupatorium lindleyanum.Scientific reports · 2025Article
- Genome-wide identification and expression profiling of bHLH transcription factors associated with ferulic acid biosynthesis inFrontiers in plant science · 2025Article
- Heterologous Expression of MYB Gene (International journal of molecular sciences · 2024Article
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- Interaction of VvDELLA2 and VvCEB1 Mediates Expression of Expansion-Related Gene during GA-Induced Enlargement of Grape Fruit.International journal of molecular sciences · 2023Article
- Impact of High Light Intensity and Low Temperature on the Growth and Phenylpropanoid Profile ofInternational journal of molecular sciences · 2023Article
- Genome-Wide Identification of bHLH Transcription Factor Family inPlants (Basel, Switzerland) · 2023Article
- Integrated transcriptome and metabolome analysis revealed that flavonoids enhanced the resistance ofFrontiers in plant science · 2023Article
- Designing plant flavonoids: harnessing transcriptional regulation and enzyme variation to enhance yield and diversity.Frontiers in plant science · 2023Review
- Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Flavonoid Accumulation and Its Underlying Mechanism in Fruits of DistinctFoods (Basel, Switzerland) · 2022Article
- Genome-wide identification of bHLH transcription factors: Discovery of a candidate regulator related to flavonoid biosynthesis inFrontiers in plant science · 2022Article
- Integrative Analysis of the Transcriptome and Metabolome Reveals Genes Involved in Phenylpropanoid and Flavonoid Biosynthesis in theFrontiers in plant science · 2022Article
- Systematic Analysis of bHLH Transcription Factors in Cassava Uncovers Their Roles in Postharvest Physiological Deterioration and Cyanogenic Glycosides Biosynthesis.Frontiers in plant science · 2022Article
- Flavonoids Accumulation in Fruit Peel and Expression Profiling of Related Genes in Purple (Plants (Basel, Switzerland) · 2021Article
- Genome-wide identification, phylogeny, and expression analysis of the bHLH gene family in tobacco (Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Regulatory Mechanisms of bHLH Transcription Factors in Plant Adaptive Responses to Various Abiotic Stresses.Frontiers in plant science · 2021Review
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9 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe basic helix-loop-helix (bHLH) transcription factors (TFs), as one of the largest families of TFs, play important roles in the regulation of many secondary metabolites including flavonoids. Their involvement in flavonoids synthesis is well established in vascular plants, but not as yet in the bryophytes. In liverworts, both bisbibenzyls and flavonoids are derived through the phenylpropanoids pathway and share several upstream enzymes.
resultsIn this study, we cloned and characterized the function of PabHLH1, a bHLH family protein encoded by the liverworts species Plagiochasma appendiculatum. PabHLH1 is phylogenetically related to the IIIf subfamily bHLHs involved in flavonoids biosynthesis. A transient expression experiment showed that PabHLH1 is deposited in the nucleus and cytoplasm, while the yeast one hybrid assay showed that it has transactivational activity. When PabHLH1 was overexpressed in P. appendiculatum thallus, a positive correlation was established between the content of bibenzyls and flavonoids and the transcriptional abundance of corresponding genes involved in the biosynthesis pathway of these compounds. The heterologous expression of PabHLH1 in Arabidopsis thaliana resulted in the activation of flavonoids and anthocyanins synthesis, involving the up-regulation of structural genes acting both early and late in the flavonoids synthesis pathway. The transcription level of PabHLH1 in P. appendiculatum thallus responded positively to stress induced by either exposure to UV radiation or treatment with salicylic acid.
conclusionPabHLH1 was involved in the regulation of the biosynthesis of flavonoids as well as bibenzyls in liverworts and stimulated the accumulation of the flavonols and anthocyanins in Arabidopsis.
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