ArticleBMC genomics2019
A transcriptomic profile of topping responsive non-coding RNAs in tobacco roots (Nicotiana tabacum).
Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Non-coding RNA: a new perspective on the regulation of secondary metabolites in medicinal plants.Molecular horticulture · 2026Review
- Topping influences crop photosynthesis and alters the absorption and redistribution of nutrients: a case study of tobacco.Frontiers in plant science · 2025Article
- Novel Differentially Expressed LncRNAs Regulate Artemisinin Biosynthesis inLife (Basel, Switzerland) · 2024Article
- Review
- Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids.Journal of experimental botany · 2024Review
- Proteomic and metabolomic insights into the impact of topping treatment on cigar tobacco.Frontiers in plant science · 2024Article
- Identification, biogenesis, function, and mechanism of action of circular RNAs in plants.Plant communications · 2023Review
- Genome-wide identification and analysis of the invertase gene family in tobacco (Frontiers in plant science · 2023Article
- Systematic Identification of Methyl Jasmonate-Responsive Long Noncoding RNAs and Their Nearby Coding Genes Unveils Their Potential Defence Roles in Tobacco BY-2 Cells.International journal of molecular sciences · 2022Article
- Comprehensive analysis of the carboxylesterase gene reveals thatFrontiers in plant science · 2022Article
- Genome-Wide Identification and Analysis of the Class III Peroxidase Gene Family in Tobacco (Frontiers in genetics · 2022Article
- Comprehensive Analysis of Long Non-coding RNA Modulates Axillary Bud Development in Tobacco (Frontiers in plant science · 2022Article
- Investigating nicotine pathway-related long non-coding RNAs in tobacco.Frontiers in genetics · 2022Article
- Article
- Circular RNAs acting as ceRNAs mediated by miRNAs may be involved in the synthesis of soybean fatty acids.Functional & integrative genomics · 2021Article
- Article
- Transcriptome analysis reveals key genes involved in the regulation of nicotine biosynthesis at early time points after topping in tobacco (Nicotiana tabacum L.).BMC plant biology · 2020Article
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 3 countries.
Funding
Abstract
backgroundNon-coding RNAs (ncRNAs), including microRNAs (miRNAs), long ncRNAs (lncRNAs) and circular RNAs (circRNAs), accomplish remarkable variety of biological functions. However, the composition of ncRNAs and their interactions with coding RNAs in modulating and controlling of cellular process in plants is largely unknown. Using a diverse group of high-throughput sequencing strategies, the mRNA, miRNA, lncRNA and circRNA compositions of tobacco (Nicotiana tabacum) roots determined and their alteration and potential biological functions in response to topping treatment analyzed.
resultsA total of 688 miRNAs, 7423 non-redundant lncRNAs and 12,414 circRNAs were identified, among which, some selected differentially expressed RNAs were verified by quantitative real-time PCR. Using the differentially expressed RNAs, a co-expression network was established that included all four types of RNAs. The number of circRNAs identified were higher than that of miRNAs and lncRNAs, but only two circRNAs were present in the co-expression network. LncRNAs appear to be the most active ncRNAs based on their numbers presented in the co-expression network, but none of them seems to be an eTM (endogenous Target Mimicry) of miRNAs. Integrated with analyses of sequence interaction, several mRNA-circRNA-miRNA interaction networks with a potential role in the regulation of nicotine biosynthesis were uncovered, including a QS-circQS-miR6024 interaction network. In this network miR6024 was significantly down-regulated, while the expression levels of its two targets, circQS and its host gene QS, were sharply increased following the topping treatment.
conclusionsThese results illustrated the transcriptomic profiles of tobacco roots, the organ responsible for nicotine biosynthesis. mRNAs always play the most important roles, while ncRNAs are also expressed extensively for topping treatment response, especially circRNAs are the most activated in the ncRNA pool. These studies also provided insights on the coordinated regulation module of coding and non-coding RNAs in a single plant biological sample. The findings reported here indicate that ncRNAs appear to form interaction complex for the regulation of stress response forming regulation networks with transcripts involved in nicotine biosynthesis in tobacco.
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