Evidence map›Paper›PMID 31726754›Full record

ArticleInternational journal of molecular sciences2019

Key Factors for A One-Pot Enzyme Cascade Synthesis of High Molecular Weight Hyaluronic Acid.

Johannes Gottschalk, Henning Zaun, Anna Eisele, Jürgen Kuballa, Lothar Elling

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 28 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Johannes GottschalkLaboratory for Biomaterials, Institute of Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Pauwelsstraße 20, 52074 Aachen, Germany.
Henning ZaunResearch and Development Department, GALAB Laboratories GmbH, Am Schleusengraben 7, 21029 Hamburg, Germany.
Anna EiseleLaboratory for Biomaterials, Institute of Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Pauwelsstraße 20, 52074 Aachen, Germany.
Jürgen KuballaResearch and Development Department, GALAB Laboratories GmbH, Am Schleusengraben 7, 21029 Hamburg, Germany.
Lothar EllingLaboratory for Biomaterials, Institute of Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Pauwelsstraße 20, 52074 Aachen, Germany.
RWTH Aachen University · DEGALAB Laboratories (Germany) · DE

Funding

Bundesministerium für Forschung und Technologie KMU-Innovativ-17 project: grant number 031B0104B
6 · The paper itself

Abstract

In the last decades, interest in medical or cosmetic applications of hyaluronic acid (HA) has increased. Size and dispersity are key characteristics of biological function. In contrast to extraction from animal tissue or bacterial fermentation, enzymatic in vitro synthesis is the choice to produce defined HA. Here we present a one-pot enzyme cascade with six enzymes for the synthesis of HA from the cheap monosaccharides glucuronic acid (GlcA) and

Indexed as

ArabidopsisArabidopsis ProteinsBacterial ProteinsHyaluronan SynthasesHyaluronic AcidKineticsPasteurella multocidaUridine Diphosphate Glucuronic AcidArabidopsis ProteinsBacterial ProteinsHyaluronan SynthasesHyaluronic AcidUridine Diphosphate Glucuronic Acidenzyme cascadehyaluronic acidin vitro synthesisone-pot multi-enzymeoptimization

Identifiers

PMID31726754
PMCPMC6888640
OpenAlexW2987381631

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.