ArticleMicrobiologyOpen2020
Combining culture and culture-independent methods reveals new microbial composition of halitosis patients' tongue biofilm.
Article in MicrobiologyOpen, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 42 citations in OpenAlex.
- Direct microbiota profiling of apheresis-associated products for microbiological insights in cell therapy.Scientific reports · 2026Article
- Effects of Trehalose on Halitosis: A Randomized Cross-Over Clinical Trial.Healthcare (Basel, Switzerland) · 2025Article
- Evaluation of the Oral Microbiome before and after Treatments for Halitosis with Photodynamic Therapy and Probiotics-Pilot Study.Healthcare (Basel, Switzerland) · 2024Article
- Unveiling the complexity of early childhood caries:Journal of oral microbiology · 2024Review
- The Oral Microbiome Profile of Water Polo Players Aged 16-20.Sports (Basel, Switzerland) · 2023Article
- Altered oral microbiome in Sudanese Toombak smokeless tobacco users carries a newly emerging risk of squamous cell carcinoma development and progression.Scientific reports · 2023Article
- Examining the Composition of the Oral Microbiota as a Tool to Identify Responders to Dietary Changes.Nutrients · 2022Article
- Site-tropism of streptococci in the oral microbiome.Molecular oral microbiology · 2022Article
- The sampling strategy of oral microbiome.iMeta · 2022Article
- Phenotypic Adaptation to Antiseptics and Effects on Biofilm Formation Capacity and Antibiotic Resistance in Clinical Isolates of Early Colonizers in Dental Plaque.Antibiotics (Basel, Switzerland) · 2022Article
- The effects of cigarette smoking on the salivary and tongue microbiome.Clinical and experimental dental research · 2022Article
- Porphyromonas gingivalis outside the oral cavity.Odontology · 2022Review
- Microbiome of Unilateral Chronic Rhinosinusitis: A Controlled Paired Analysis.International journal of environmental research and public health · 2021Article
- The role of oral microbiome in pemphigus vulgaris.Archives of microbiology · 2021Article
- Identification of Salivary Microorganisms and Metabolites Associated with Halitosis.Metabolites · 2021Article
- Structural Equation Modeling of Tongue Function and Tongue Hygiene in Acute Stroke Patients.International journal of environmental research and public health · 2021Article
- A Log Ratio-Based Analysis of Individual Changes in the Composition of the Oral Microbiota in Different Dietary Phases.Nutrients · 2021Article
- Effects of a derivative of reutericin 6 and gassericin A on the biofilm of Streptococcus mutans in vitro and caries prevention in vivo.Odontology · 2021Article
- A Literature Review and Framework Proposal for Halitosis Assessment in Cigarette Smokers and Alternative Nicotine-Delivery Products Users.Frontiers in oral health · 2021Review
- Dietary Factors Affecting the Prevalence and Impact of Periodontal Disease.Clinical, cosmetic and investigational dentistry · 2021Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOral malodor is a very discomforting condition deriving from the presence of volatile sulfur compounds in the expired air. In halitosis of intraoral etiology, the volatile sulfur compounds are metabolic products of the oral microorganisms within the biofilm coating the tongue dorsum as well as other tissues in the oral cavity. The aim of this study was to characterize and compare the microbial composition of tongue biofilm in volunteers suffering from halitosis and healthy volunteers by means of both the culture method and culture-independent cloning technique.
resultsA high bacterial variety (more than 80 different species) was detected using the combination of both methods. A distinct bacterial composition was revealed in the halitosis-associated biofilms compared with the health-associated biofilms. Actinomyces graevenitzii was shown to be significantly associated with the halitosis condition. The culture method identified 47 species, included Veillonella rogosae, never isolated from the tongue biofilm of halitosis patients so far. In the healthy condition, the culture-dependent method showed that the most frequent species were Streptococcus parasanguinis among the aerobes and Veillonella spp. among the anaerobes. The culture-independent cloning method detected more than 50 species. Streptococci, in particular S. mitis/oralis, S. pseudopneumoniae, and S. infantis as well as Prevotella spp., were found most frequently in halitosis patients. Streptococcus salivarius and Rothia mucilaginosa were found more frequently in the healthy condition.
conclusionsThe combination of the culture-dependent and culture-independent cloning techniques allowed for a widespread analysis of the tongue biofilm in halitosis patients. The results can support further pharmacological research for new antimicrobial agents and halitosis therapy strategies.
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