Evidence map›Paper›PMID 31725199›Full record

ArticleJournal of cellular and molecular medicine2020

l-tetrahydropalmatine suppresses osteoclastogenesis in vivo and in vitro via blocking RANK-TRAF6 interactions and inhibiting NF-κB and MAPK pathways.

Xin Zhi, Lipeng Wang, Huiwen Chen, Chao Fang, Jin Cui, Yan Hu, Liehu Cao, Weizong Weng, Qirong Zhou, Longjuan Qin and 8 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 36 citations in OpenAlex.

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  12. 1α,25-(OH)In vitro cellular & developmental biology. Animal · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 2 institutions in 2 countries.

Xin ZhiDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Lipeng WangGraduate Management Unit, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Huiwen ChenDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Chao FangDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Jin CuiDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Yan HuDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Liehu CaoDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Weizong WengDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Qirong ZhouDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Longjuan QinOrthopedic Basic and Translational Research Center, Jiangyin, China.
Hongyuan SongDepartment of Ophthalmology, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Yajun WangDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Yao WangDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Hao JiangDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Xiaoqun LiDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Sicheng WangDepartment of Orthopedics, Shanghai Zhongye Hospital, Shanghai, China.
Xiao ChenDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Jiacan SuDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.ORCID 0000-0001-7080-263X
Second Military Medical University · CNZhongshan Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bone homeostasis is delicately orchestrated by osteoblasts and osteoclasts. Various pathological bone loss situations result from the overactivated osteoclastogenesis. Receptor activator of nuclear factor κB ligand (RANKL)-activated NF-κB and MAPK pathways is vital for osteoclastogenesis. Here, we for the first time explored the effects of l-tetrahydropalmatine (l-THP), an active alkaloid derived from corydalis, on the formation and function of osteoclasts in vitro and in vivo. In RAW264.7 cells and bone marrow monocytes cells (BMMCs), l-THP inhibited osteoclastic differentiation at the early stage, down-regulated transcription level of osteoclastogenesis-related genes and impaired osteoclasts functions. Mechanically, Western blot showed that l-THP inhibited the phosphorylation of P50, P65, IκB, ERK, JNK and P38, and the electrophoretic mobility shift assay (EMSA) revealed that DNA binding activity of NF-κB was suppressed, ultimately inhibiting the expression of nuclear factor of activated T cells (NFATc1). Besides, Co-immunoprecipitation indicated that l-THP blocked the interactions of RANK and TNF receptor associated factor 6 (TRAF6) at an upstream site. In vivo, l-THP significantly inhibited ovariectomy-induced bone loss and osteoclastogenesis in mice. Collectively, our study demonstrated that l-THP suppressed osteoclastogenesis by blocking RANK-TRAF6 interactions and inhibiting NF-κB and MAPK pathways. l-THP is a promising agent for treating osteoclastogenesis-related diseases such as post-menopausal osteoporosis.

Indexed as

OsteogenesisAnimalsAnti-Arrhythmia AgentsBerberine AlkaloidsBone ResorptionCell DifferentiationFemaleIn Vitro TechniquesMiceMice, Inbred C57BLMitogen-Activated Protein KinasesNF-kappa BOsteoblastsProtein Interaction Domains and MotifsReceptor Activator of Nuclear Factor-kappa BTNF Receptor-Associated Factor 6Anti-Arrhythmia AgentsBerberine AlkaloidsMitogen-Activated Protein KinasesNF-kappa BReceptor Activator of Nuclear Factor-kappa BtetrahydropalmatineTNF Receptor-Associated Factor 6Tnfrsf11a protein, mouseTRAF6 protein, mousel-tetrahydropalmatineosteoclastogenesisosteoporosisRANKL

Identifiers

PMID31725199
PMCPMC6933417
OpenAlexW2986061796

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.