ArticleNature protocols2019
High spatial resolution imaging of biological tissues using nanospray desorption electrospray ionization mass spectrometry.
Article in Nature protocols, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
105 citing papers in PubMed, 211 citations in OpenAlex.
- Spatially resolved lipid compartmentalization in human pancreatic islets revealed by high-resolution ultra-low-flow-rate desorption electrospray ionization mass spectrometry imaging.Analytical and bioanalytical chemistry · 2026Article
- Single-cell mass spectrometry imaging: platform advances for multimodal spatial omics.Analytical and bioanalytical chemistry · 2026Review
- Fabrication of thermoplastic microfluidic probes for nanospray desorption electrospray ionization mass spectrometry.International journal of mass spectrometry · 2026Article
- Enhancing IR-MALDESI MSI Spatial Resolution Through Beam Constriction With a Ring-Actuated Iris.Rapid communications in mass spectrometry : RCM · 2026Article
- Development of a Tapping-Mode Scanning Probe Electrospray Ionization Platform for High-Sensitivity and Long-Term Stability in Single-Cell Mass Spectrometry Imaging of Tissue.Analytical chemistry · 2026Article
- Living single-cell metabolomicsChemical science · 2026Review
- Benchmarking Spatial Clustering Methods for Mass Spectrometry-Based Spatial Metabolomics.Metabolites · 2026Article
- Multiplexed Tandem Mass Spectrometry Imaging Enables Large-Scale Isomer Mapping and Annotation in Tissues.Angewandte Chemie (International ed. in English) · 2026Article
- Probing Proteoform Heterogeneity From Single Human Oocytes.Molecular & cellular proteomics : MCP · 2026Article
- Achieving Single-Cell Resolution via Desorption Electrospray Ionization Mass Spectrometry Imaging (DESI-MSI) on Different Platforms.Analytical chemistry · 2026Article
- Single-Cell and Spatial Omics: Methods and Applications.MedComm · 2026Review
- Proteoform profiling of endogenous single cells from rat hippocampus at scale.Nature biotechnology · 2026Article
- Imaging bioactive lipid isomers in acetaminophen-induced liver injury using nano-DESI tandem MS.Journal of lipid research · 2026Article
- Mathematical Framework for Quantifying Delocalization in MALDI-MSI via a Composite Scoring Approach.ACS measurement science au · 2026Article
- Integrated Native Mass Spectrometry Imaging of Soluble and Membrane Proteins.Journal of the American Chemical Society · 2026Article
- Quantitative Mass Spectrometry Imaging of Intact Proteins in Tissues.Analytical chemistry · 2026Article
- Research Progress on the Application of Mass Spectrometry Imaging Technology in Cerebral Disease.Life (Basel, Switzerland) · 2026Review
- High-spatial-resolution mass spectrometry imaging of biological tissues using a microfluidic probe.Nature protocols · 2026Review
- Imaging of Protein Assemblies up to 231 kDa in Tissues with Nano-DESI Mass Spectrometry.Analytical chemistry · 2025Article
- Topological segmentation of mass spectrometry imaging data.Journal of mass spectrometry and advances in the clinical lab · 2025Article
45 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Mass spectrometry imaging (MSI) enables label-free spatial mapping of hundreds of biomolecules in tissue sections. This capability provides valuable information on tissue heterogeneity that is difficult to obtain using population-averaged assays. Despite substantial developments in both instrumentation and methodology, MSI of tissue samples at single-cell resolution remains challenging. Herein, we describe a protocol for robust imaging of tissue sections with a high (better than 10-μm) spatial resolution using nanospray desorption electrospray ionization (nano-DESI) mass spectrometry, an ambient ionization technique that does not require sample pretreatment before analysis. In this protocol, mouse uterine tissue is used as a model system to illustrate both the workflow and data obtained in these experiments. We provide a detailed description of the nano-DESI MSI platform, fabrication of the nano-DESI and shear force probes, shear force microscopy experiments, spectral acquisition, and data processing. A properly trained researcher (e.g., technician, graduate student, or postdoc) can complete all the steps from probe fabrication to data acquisition and processing within a single day. We also describe a new strategy for acquiring both positive- and negative-mode imaging data in the same experiment. This is achieved by alternating between positive and negative data acquisition modes during consecutive line scans. Using our imaging approach, hundreds of high-quality ion images were obtained from a single uterine section. This protocol enables sensitive and quantitative imaging of lipids and metabolites in heterogeneous tissue sections with high spatial resolution, which is critical to understanding biochemical processes occurring in biological tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.