ArticleScientific reports2019
Pipetting-based immunoassay for point-of-care testing: Application for detection of the influenza A virus.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Micro-Analytical Lab-on-a-Tip: Advances and Perspectives.Analytical chemistry · 2025Review
- Article
- Trends of respiratory virus detection in point-of-care testing: A review.Analytica chimica acta · 2023Review
- Engineering Innovative Interfaces for Point-of-Care Diagnostics.Current opinion in colloid & interface science · 2023Review
- Sample-to-Answer Immuno-Magnetic Assay Using Thermally Responsive Alkane Partitions.Biosensors · 2022Article
- Thermally Responsive Alkane Partitions for Assay Automation.ACS applied materials & interfaces · 2022Article
- Mag-spinner: a next-generation Facile, Affordable, Simple, and porTable (FAST) magnetic separation system.Nanoscale advances · 2022Article
- Nanomaterials for IoT Sensing Platforms and Point-of-Care Applications in South Korea.Sensors (Basel, Switzerland) · 2022Review
- Specific and sensitive detection of Influenza A virus using a biotin-coated nanoparticle enhanced immunomagnetic assay.Analytical and bioanalytical chemistry · 2022Article
- Recent Progress on Rapid Lateral Flow Assay-Based Early Diagnosis of COVID-19.Frontiers in bioengineering and biotechnology · 2022Review
- Fluorescent paper strip immunoassay with carbon nanodots@silica for determination of human serum amyloid A1.Mikrochimica acta · 2021Article
- A critical review of point-of-care diagnostic technologies to combat viral pandemics.Analytica chimica acta · 2021Review
- SERS-Based Colloidal Aptasensors for Quantitative Determination of Influenza Virus.International journal of molecular sciences · 2021Article
- Small-volume point-of-care analytical methods.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
13 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Point-of-care tests (POCT) for pathogens are considered important for low-resource countries and facilities. Although lateral flow immunoassays (LFIA) have many advantages including speed and ease of use, their sensitivity is limited without specific equipment. Furthermore, their response cannot be enhanced through enzymatic reactions. Owing to these limitations, LFIAs have not yet been generally adopted as the standard protocol for in vitro analysis of infectious pathogens. We aimed to develop a novel pipetting-based immunoassay using a removable magnetic ring-coupled pipette tip. The "magnetic bead-capture antibody-targeted protein complex" was simply purified by pipetting and quantified by enzymatic colour development or using a lateral flow system. This pipetting-based immunoassay was applied to detect the nucleoprotein (NP) of the influenza A virus. Using an HRP-conjugated monoclonal antibody as a probe, the assay allowed for specific and sensitive detection. Furthermore, when this assay was applied exclusively for antigen capture in the lateral flow system, the limit of detection improved 100-fold and displayed greater sensitivity than the lateral flow system alone. Therefore, the pipetting-based immunoassay may be potentially used as a sensitive POCT to clinically detect a target antigen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.